Binding Constant of V IV O to Transferrin
| العنوان: | Binding Constant of V IV O to Transferrin |
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| المؤلفون: | Hiromu Sakurai, Saâd Bouhsina, Éva A. Enyedy, Tamás Jakusch, Tamás Kiss |
| المصدر: | European Journal of Inorganic Chemistry. 2006:3607-3613 |
| بيانات النشر: | Wiley, 2006. |
| سنة النشر: | 2006 |
| مصطلحات موضوعية: | Chemistry, Analytical chemistry, Nitrilotriacetic acid, Free-energy relationship, Ligand (biochemistry), Binding constant, law.invention, Inorganic Chemistry, chemistry.chemical_compound, Ultraviolet visible spectroscopy, law, Physical chemistry, Taft equation, Electron paramagnetic resonance, Ternary complex |
| الوصف: | The interactions of bis-VIVO-1,2-dimethyl-3-hydroxy-4(1H)-pyridinone (DHP) with apotransferrin (apoTF) were followed by means of electron paramagnetic resonance spectroscopy (EPR) at room temperature and at liquid nitrogen temperature. Partial ligand displacement between DHP and apoTF was observed, and the reaction was used to determine the binding constant of VIVO to apoTF. A value of log K = 14.3 ± 0.6 was obtained for the binding of the first VIVO to apoTF. In order to confirm the validity of the binding constant, UV spectroscopy was used to monitor the displacement reaction between VIVO–apoTF and nitrilotriacetic acid (NTA). The difference between the binding constants obtained by the two different methods (ca. one log unit) was explained in terms of the possible ternary complex formation between VIVO–apoTF and the displacing ligand. The binding constants obtained exhibit a reasonably good agreement with the linear free energy relationship (LFER) estimation. (© Wiley-VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2006) |
| تدمد: | 1099-0682 1434-1948 |
| DOI: | 10.1002/ejic.200600314 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::ff46b926682336a91ce04987ac484599 https://doi.org/10.1002/ejic.200600314 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........ff46b926682336a91ce04987ac484599 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10990682 14341948 |
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| DOI: | 10.1002/ejic.200600314 |