Local cyclical compression modulates macrophage functionin situand alleviates immobilization-induced muscle atrophy
| العنوان: | Local cyclical compression modulates macrophage functionin situand alleviates immobilization-induced muscle atrophy |
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| المؤلفون: | Atsushi Takashima, Keisuke Sawada, Yoshinori Ichihara, Motoshi Nagao, Karina Kouzaki, Toru Ogata, Takahiro Maekawa, Noriaki Kawanishi, Koichi Nakazato, Hideki Nakamoto, Ichiro Harada, Youngjae Ryu, Yasuhiro Sawada, Michiru Makuuchi, Shunsuke Kawamura, Daisuke Yoshino, Katsuhiko Suzuki, Naoyoshi Sakitani, Masakuni Tokunaga, Kumiko Saitou, Taku Saito |
| المصدر: | Clinical Science. 132:2147-2161 |
| بيانات النشر: | Portland Press Ltd., 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, business.industry, Monocyte, Hydrostatic pressure, Inflammation, General Medicine, Muscle atrophy, Cell biology, 03 medical and health sciences, 030104 developmental biology, medicine.anatomical_structure, Interstitial fluid, Medicine, Macrophage, Myocyte, Tumor necrosis factor alpha, medicine.symptom, business |
| الوصف: | Physical inactivity gives rise to numerous diseases and organismal dysfunctions, particularly those related to aging. Musculoskeletal disorders including muscle atrophy, which can result from a sedentary lifestyle, aggravate locomotive malfunction and evoke a vicious circle leading to severe functional disruptions of vital organs such as the brain and cardiovascular system. Although the significance of physical activity is evident, molecular mechanisms behind its beneficial effects are poorly understood. Here, we show that massage-like mechanical interventions modulate immobilization-induced pro-inflammatory responses of macrophages in situ and alleviate muscle atrophy. Local cyclical compression (LCC) on mouse calves, which generates intramuscular pressure waves with amplitude of 50 mmHg, partially restores the myofiber thickness and contracting forces of calf muscles that are decreased by hindlimb immobilization. LCC tempers the increase in the number of cells expressing pro-inflammatory proteins, tumor necrosis factor-α and monocyte chemoattractant protein-1 (MCP-1), including macrophages in situ. The reversing effect of LCC on immobilization-induced thinning of myofibers is almost completely nullified when macrophages recruited from circulating blood are depleted by administration of clodronate liposomes. Furthermore, application of pulsatile fluid shear stress, but not hydrostatic pressure, reduces the expression of MCP-1 in macrophages in vitro. Together with the LCC-induced movement of intramuscular interstitial fluid detected by µCT analysis, these results suggest that mechanical modulation of macrophage function is involved in physical inactivity-induced muscle atrophy and inflammation. Our findings uncover the implication of mechanosensory function of macrophages in disuse muscle atrophy, thereby opening a new path to develop a novel therapeutic strategy utilizing mechanical interventions. |
| تدمد: | 1470-8736 0143-5221 |
| DOI: | 10.1042/cs20180432 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::f50e05f6fec359840e54ee93d5becb2c https://doi.org/10.1042/cs20180432 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........f50e05f6fec359840e54ee93d5becb2c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14708736 01435221 |
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| DOI: | 10.1042/cs20180432 |