Application of 1H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib
| العنوان: | Application of 1H-NMR combined with qRT-PCR technology in the exploration of rosmarinic acid biosynthesis in hair roots of Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib |
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| المؤلفون: | Feng Su, Jipeng Chen, Zhuoni Hou, Yuanyuan Li, Ling Xu, Xiaodan Zhang, Zongsuo Liang, Dongfeng Yang |
| المصدر: | Planta. 253 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Methyl jasmonate, biology, Traditional medicine, Rosmarinic acid, Plant Science, Phenolic acid, biology.organism_classification, Salvia miltiorrhiza, 03 medical and health sciences, chemistry.chemical_compound, Metabolic pathway, 030104 developmental biology, 0302 clinical medicine, chemistry, Biosynthesis, 030220 oncology & carcinogenesis, Genetics, Inducer, Salvia castanea |
| الوصف: | Main conclusion Methyl jasmonate promotes the synthesis of rosmarinic acid in Salvia miltiorrhiza Bunge and Salvia castanea f. tomentosa Stib, and it promotes the latter more strongly. Abstract Salvia miltiorrhiza Bunge (SMB) is a traditional Chinese medicinal material, its water-soluble phenolic acid component rosmarinic acid has very important medicinal value. Salvia castanea f. tomentosa Stib (SCT) mainly distributed in Nyingchi, Tibet. Its pharmacological effects are similar to SMB, but its rosmarinic acid is significantly higher than the former. Methyl jasmonate (MJ) as an inducer can induce the synthesis of phenolic acids in SMB and SCT. However, the role of MJ on rosmarinic acid in SMB is controversial. Therefore, this study used SMB and SCT hair root as an experimental material and MJ as a variable. On one hand, exploring the controversial reports in SMB; on the other hand, comparing the differences in the mechanism of action of MJ on the phenolic acids in SMB and SCT. The content of related metabolites and the expression of key genes in the synthesis pathway of rosmarinic acid was analyzed by 1H-NMR combined with qRT-PCR technology. Our research has reached the following conclusions: first of all, MJ promotes the accumulation of rosmarinic acid and related phenolic acids in the metabolic pathways of SMB and SCT. After MJ treatment, the content of related components and gene expression are increased. Second, compared to SMB, SCT has a stronger response to MJ. It is speculated that the different responses of secondary metabolism-related genes to MJ may lead to different metabolic responses of salvianolic acid between the two. |
| تدمد: | 1432-2048 0032-0935 |
| DOI: | 10.1007/s00425-020-03506-y |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::f00f828103d3a184d703797e3f120da7 https://doi.org/10.1007/s00425-020-03506-y |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........f00f828103d3a184d703797e3f120da7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14322048 00320935 |
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| DOI: | 10.1007/s00425-020-03506-y |