Starch-branching enzymesSbe1 andSbe2 from wheat (Triticum aestivum cv. Cheyenne): Molecular characterization, development expression, and homoeologue assignment by differential PCR
| العنوان: | Starch-branching enzymesSbe1 andSbe2 from wheat (Triticum aestivum cv. Cheyenne): Molecular characterization, development expression, and homoeologue assignment by differential PCR |
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| المؤلفون: | Olin D. Anderson, Charlene K. Tanka, William J. Hurkman, Kent F. McCue |
| المصدر: | Plant Molecular Biology Reporter. 20:191-192 |
| بيانات النشر: | Springer Science and Business Media LLC, 2002. |
| سنة النشر: | 2002 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Genetics, Expressed sequence tag, biology, Starch, Wheat flour, food and beverages, RNA, Plant Science, Endosperm, chemistry.chemical_compound, Enzyme, chemistry, Complementary DNA, biology.protein, Starch synthase, Molecular Biology |
| الوصف: | Starch is the main component of the wheat kernel, and wheat flour is used for hundreds of food and nonfood products. We are exploring ways to improve wheat quality and to develop new uses for wheat based on altered starch characteristics. To understand the molecular basis for variations in the physical and chemical properties of starch, we examined transcripts for starch biosynthetic enzymes. cDNAs encoding 2 isoforms of starch-branching enzyme (Sbe1, Sbe2) were isolated from wheat endosperm. The longestSbe1 andSbe2 cDNAs were 2797 and 2975 bp, respectively, and they shared extensive identity withSbe sequences reported for wheat and other species. With orthologue-specific primer pairs, homoeologue assignments to chromosome 7 were made forSbe1#19 (TRIae: Sbe1A.1) andSbe1#9 (TRIae:Sbe1D.1) using the wheat cv. Chinese Spring nullisomic-tetrasomic-7 lines. This strategy may prove useful for future mapping of expressed sequence tag (EST) data. TheSbe cDNAs and a granule-bound starch synthase cDNA (GbssI) (from an EST sequencing project) were used to examine the steady-state RNA levels during development of the wheat. Steady-state levels ofSbe2 mRNA were detectable 5 d postanthesis (DPA) and reached a maximum at 10 DPA. Steady-state levels ofSbe1 andGbssI began to rise at 10 DPA and peaked at 15 DPA. Levels of all messages declined rapidly at 20–25 DPA. Reported here is the first analysis of transcripts for these enzymes in the same RNA pools and demonstration that expression patterns are unique and developmentally regulated. |
| تدمد: | 1572-9818 0735-9640 |
| DOI: | 10.1007/bf02799436 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::efe4fe2e5825878509f332b3c3c73ef9 https://doi.org/10.1007/bf02799436 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........efe4fe2e5825878509f332b3c3c73ef9 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15729818 07359640 |
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| DOI: | 10.1007/bf02799436 |