Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L
| العنوان: | Molecular cloning and characterisation of two enzymes involved in the rosmarinic acid biosynthesis pathway of Prunella vulgaris L |
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| المؤلفون: | Tianlin Pei, Zhenqing Bai, Kunru Wang, Yanyan Jia, Shaoxuan He, Liang Peng, Zongsuo Liang, Mei Ru, Hongyan Li |
| المصدر: | Plant Cell, Tissue and Organ Culture (PCTOC). 128:381-390 |
| بيانات النشر: | Springer Science and Business Media LLC, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0106 biological sciences, 0301 basic medicine, chemistry.chemical_classification, Sequence analysis, Rosmarinic acid, Prunella vulgaris, Horticulture, Monooxygenase, Biology, Molecular cloning, biology.organism_classification, 01 natural sciences, Amino acid, 03 medical and health sciences, chemistry.chemical_compound, Open reading frame, 030104 developmental biology, Biochemistry, chemistry, Biosynthesis, 010606 plant biology & botany |
| الوصف: | Prunella vulgaris, a widely-used medicinal perennial herb, contains various active compounds and has multifaceted medicinal activities. As the index component in P. vulgaris, rosmarinic acid (RA) is a typical phenolic acid, with significant anti-inflammatory, anti-oxidant, anti-tumour, anti-viral, and anti-microbial properties. To better understand the RA biosynthetic pathway in P. vulgaris, we isolated and cloned the cDNA sequences of putative RA synthase (PvRAS) and cytochrome P450 monooxygenase (PvCYP98A101), two important enzymes catalysing RA biosynthesis. Sequence analysis revealed that PvRAS contained an open reading frame (ORF) of 1305 bp encoding a 435-amino-acid residue belonging to the BAHD acyltransferase family, and PvCYP98A101 contained an ORF of 1530 bp encoding a 510-amino-acid residue, as a member of the CYP98A family. The deduced PvRAS and PvCYP98A101 amino acid sequences shared high similarity with other putative/known RASs and CYPs, respectively. Quantitative real-time PCR analysis showed that constitutive expression of PvRAS and PvCYP98A101 was much higher in roots than in leaves, stems, or spikes. Further analysis indicated that PvRAS was localised in the cytosol and nucleus, whilst PvCYP98A101 existed as a membrane protein in the endoplasmic reticulum. These results highlight the RA biosynthesis pathway in P. vulgaris and, provide useful information to engineer natural products. |
| تدمد: | 1573-5044 0167-6857 |
| DOI: | 10.1007/s11240-016-1117-z |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::ee896d32cca86a7d6fa9f22953f0d588 https://doi.org/10.1007/s11240-016-1117-z |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........ee896d32cca86a7d6fa9f22953f0d588 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15735044 01676857 |
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| DOI: | 10.1007/s11240-016-1117-z |