التفاصيل البيبلوغرافية
| العنوان: |
Convergent solid-phase and solution approaches in the synthesis of the cysteine-rich Mdm2 RING finger domain |
| المؤلفون: |
Zoe Vasileiou, Dimitrios Gatos, Kostas K. Barlos |
| المصدر: |
Journal of Peptide Science. 15:824-831 |
| بيانات النشر: |
Wiley, 2009. |
| سنة النشر: |
2009 |
| مصطلحات موضوعية: |
Pharmacology, chemistry.chemical_classification, biology, Stereochemistry, Organic Chemistry, Condensation, Convergent synthesis, Peptide, General Medicine, Biochemistry, Chemical synthesis, Combinatorial chemistry, RING finger domain, chemistry, Structural Biology, Phase (matter), Drug Discovery, biology.protein, Molecular Medicine, Mdm2, Molecular Biology, Cysteine |
| الوصف: |
The RING finger domain of the Mdm2, located at the C-terminus of the protein, is necessary for regulation of p53, a tumor suppressor protein. The 48-residues long Mdm2 peptide is an important target for studying its interaction with small anticancer drug candidates. For the chemical synthesis of the Mdm2 RING finger domain, the fragment condensation on solid-phase and the fragment condensation in solution were studied. The latter method was performed using either protected or free peptides at the C-terminus as the amino component. Best results were achieved using solution condensation where the N-component was applied with the C-terminal carboxyl group left unprotected. The developed method is well suited for large-scale synthesis of Mdm2 RING finger domain, combining the advantages of both solid-phase and solution synthesis. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd. |
| تدمد: |
1075-2617 |
| DOI: |
10.1002/psc.1182 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::ede0b0d9221c2c9cf8bc850924d58fc5
https://doi.org/10.1002/psc.1182 |
| Rights: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........ede0b0d9221c2c9cf8bc850924d58fc5 |
| قاعدة البيانات: |
OpenAIRE |