Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia)
| العنوان: | Fluorescence in situ hybridization analysis of the ALK gene in 2,045 non-small cell lung cancer patients from North-Western Spain (Galicia) |
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| المؤلفون: | Rosario García-Campelo, Francisco J. Afonso-Afonso, Margarita Amenedo, Sergio Vazquez-Estevez, Ana L. González-Piñeiro, Martín Lázaro-Quintela, Ihab Abdulkader, Juan Ruiz-Bañobre, Ángel Vázquez-Boquete, Lucía Santomé, José Cameselle-Teijeiro, Cristina Azpitarte-Raposeiras, Raquel Pérez-Becerra, Jose L. Fírvida-Pérez, María Sánchez-Ares, Yolanda Rico-Rodríguez, Elena Couso, José Antúnez, Joaquín Casal-Rubio |
| المصدر: | Oncology Letters. 12:1403-1407 |
| بيانات النشر: | Spandidos Publications, 2016. |
| سنة النشر: | 2016 |
| مصطلحات موضوعية: | 0301 basic medicine, Cancer Research, Pathology, medicine.medical_specialty, Oncogene, medicine.diagnostic_test, Cancer, Biology, medicine.disease, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Oncology, hemic and lymphatic diseases, 030220 oncology & carcinogenesis, Cytology, Biopsy, medicine, Anaplastic lymphoma kinase, Adenocarcinoma, Lung cancer, Fluorescence in situ hybridization |
| الوصف: | Identification of anaplastic lymphoma receptor tyrosine kinase (ALK) gene rearrangements is a standard diagnostic test in patients with advanced non-small cell lung cancer (NSCLC). The current study describes the experience of ALK rearrangement detection of a referral center in the public health care system of Galicia in North-Western Spain. The fluorescence in situ hybridization (FISH) patterns of the ALK gene and the clinical and pathological features of these patients are reported. This study is also of interest for comparative purposes due to the relative geographical isolation of the area, which could have contributed to particular genetic features. A total of 2,045 tissue samples from NSCLC patients were collected between October 2010 and July 2015 and tested for ALK rearrangements by FISH. Examination of 1,686 paraffin-embedded tissue specimens and 395 cytological samples (306 cell block preparations and 53 cytological smears) was conducted, and any associations between the FISH results and clinicopathological features were assessed. The rate of successful evaluation was marginally higher in tissue samples than in cytological samples (92.9% vs. 84.1%); this difference was not significant. ALK rearrangements were identified in 82 patients(4%): 65 (79.3%) in tissue specimens, 15 (18.3%) in cell block samples and 2 (2.4%) in cytological smears. This genetic translocation appeared to be associated with a non-smoking history, younger age, female gender, stage IV and adenocarcinoma histological type. The findings demonstrate that ALK evaluation by FISH is feasible in tissue and cytological samples. The clinical and pathological features of the ALK-positive series of patients are similar to those previously reported in the literature. |
| تدمد: | 1792-1082 1792-1074 |
| DOI: | 10.3892/ol.2016.4788 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::eb3329cff9ab9e656216a11c1f567199 https://doi.org/10.3892/ol.2016.4788 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........eb3329cff9ab9e656216a11c1f567199 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17921082 17921074 |
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| DOI: | 10.3892/ol.2016.4788 |