التفاصيل البيبلوغرافية
| العنوان: |
Early stages of induction of anterior head ectodermal properties inXenopusembryos are mediated by transcriptional cofactorldb1 |
| المؤلفون: |
Carol Zygar Plautz, Malia J. Deshotel, Robert M. Grainger, Brett Zirkle |
| المصدر: |
Developmental Dynamics. 243:1606-1618 |
| بيانات النشر: |
Wiley, 2014. |
| سنة النشر: |
2014 |
| مصطلحات موضوعية: |
Gene knockdown, Xenopus, Neural crest, Ectoderm, Biology, biology.organism_classification, Molecular biology, medicine.anatomical_structure, Lens (anatomy), Expression cloning, Eye development, medicine, Neural plate, Developmental Biology |
| الوصف: |
Background: Specific molecules involved in early inductive signaling from anterior neural tissue to the placodal ectoderm to establish a lens-forming bias, as well as their regulatory factors, remain largely unknown. In this study, we sought to identify and characterize these molecules. Results: Using an expression cloning strategy to isolate genes with lens-inducing activity, we identified the transcriptional cofactor ldb1. This, together with evidence for its nuclear dependence, suggests its role as a regulatory factor, not a direct signaling molecule. We propose that ldb1 mediates induction of early lens genes in our functional assay by transcriptional activation of lens-inducing signals. Gain-of-function assays demonstrate that the inductive activity of the anterior neural plate on head ectodermal structures can be augmented by ldb1. Loss-of-function assays show that knockdown of ldb1 leads to decreased expression of early lens and retinal markers and subsequently to defects in eye development. Conclusions: The functional cloning, expression pattern, overexpression, and knockdown data show that an ldb1-regulated mechanism acts as an early signal for Xenopus lens induction. Developmental Dynamics 243:1606–1618, 2014. © 2014 Wiley Periodicals, Inc. |
| تدمد: |
1058-8388 |
| DOI: |
10.1002/dvdy.24193 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::eb2ad84f30f110d440632ac96ef6cb17
https://doi.org/10.1002/dvdy.24193 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi...........eb2ad84f30f110d440632ac96ef6cb17 |
| قاعدة البيانات: |
OpenAIRE |