Platelet-rich plasma (PRP) is currently used in regenerative medicine and has opened up an exciting field of study. In this chapter, we present the main challenges limiting the study and standardization of PRP, based on variables and interactions involved in its preparation. Furthermore, we present and discuss some approaches to basic science studies of PRP from the simplest case to the most complex or real scenario. A feasible strategy for studying P-PRP (Pure-PRP) preparation in a real situation is also illustrated. The key point of the strategy is the predictive description of the main steps of PRP preparation, based on the physicochemical events. Within the expected variability for an autologous product, the quality of PRP can be modulated by preparation conditions and also tailored for evaluation of biological responses. The interconnected characterization and standardization of the quality and biological properties of PRP are the basis for further clinical studies