Design for preparation of more active cross-linked enzyme aggregates of Burkholderia cepacia lipase using palm fiber residue
| العنوان: | Design for preparation of more active cross-linked enzyme aggregates of Burkholderia cepacia lipase using palm fiber residue |
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| المؤلفون: | Álvaro S. Lima, Raquel L. C. Giordano, Nanda R Alves, Ranyere Lucena de Souza, Matheus M. Pereira, Cleide Mara Faria Soares, Paulo Waldir Tardioli |
| المصدر: | Bioprocess and Biosystems Engineering. 44:57-66 |
| بيانات النشر: | Springer Science and Business Media LLC, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, chemistry.chemical_classification, biology, 010405 organic chemistry, Chemistry, Supramolecular chemistry, Bioengineering, General Medicine, engineering.material, biology.organism_classification, 01 natural sciences, 0104 chemical sciences, Residue (chemistry), Hydrolysis, Burkholderia, Enzyme, Coating, 010608 biotechnology, engineering, biology.protein, Organic chemistry, Industrial and production engineering, Lipase, Biotechnology |
| الوصف: | A new design of cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase (BCL) based mainly on the use of lignocellulosic residue of palm fiber as an additive was proposed. Different parameters for the preparation of active CLEAs in the hydrolysis of olive oil, such as precipitation agents, crosslinking agent concentration, additives, and coating agents were investigated. The highest activity yield (121.1 ± 0.1%) and volumetric activity (1578.1 ± 2.5 U/mL) were achieved for CLEAs prepared using the combination of a coating step with Triton® X-100 and polyethyleneimine plus the use of palm fiber as an additive. The variations of the secondary structures of BCL-CLEAs were analyzed by second-derivative infrared spectra, mainly indicating a reduction of the α-helix structure, which was responsible for the lipase activation in the supramolecular structure of the CLEAs. Thus, these results provided evidence of an innovative design of BCL-CLEAs as a sustainable and biocompatible opportunity for biotechnology applications. |
| تدمد: | 1615-7605 1615-7591 |
| DOI: | 10.1007/s00449-020-02419-0 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e8ad0de25a3eaca2e04a900fb134b3e5 https://doi.org/10.1007/s00449-020-02419-0 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........e8ad0de25a3eaca2e04a900fb134b3e5 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16157605 16157591 |
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| DOI: | 10.1007/s00449-020-02419-0 |