Evaluation of Lipid-polyethylenimine Conjugates as Biocompatible Carriers of CpG Oligodeoxynucleotides to Macrophages
| العنوان: | Evaluation of Lipid-polyethylenimine Conjugates as Biocompatible Carriers of CpG Oligodeoxynucleotides to Macrophages |
|---|---|
| المؤلفون: | Jiwon Yang, Gayeon You, Eun Seo Choi, Hyejung Mok |
| المصدر: | Biotechnology and Bioprocess Engineering. 26:586-594 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Polyethylenimine, Chemistry, CpG Oligodeoxynucleotide, technology, industry, and agriculture, Biomedical Engineering, Bioengineering, macromolecular substances, Polyethylene glycol, Applied Microbiology and Biotechnology, chemistry.chemical_compound, Immune system, Biochemistry, CpG site, Cytokine secretion, Cytotoxicity, Biotechnology, Conjugate |
| الوصف: | Considering the potent immune stimulation by CpG oligodeoxynucleotides (CpGs), the development of CpG carriers is a prerequisite for efficient cancer immunotherapy. In this study, we conjugated 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[hydroxyl succinimidyl (polyethylene glycol)] (DSPE-PEG-NHS) with polyethylenimine (PEI) to develop a PEI-PEG-DSPE conjugate that can serve as a biocompatible and efficient CpG carrier. Five types of PEIPEG-DSPE conjugates were developed, each with different molecular weights of PEI and different degrees of DSPEPEG modification, and all exhibited significantly lower cytotoxicity. In particular, compared to CpG delivery via natural PEI, delivery with PEI (25 kDa)-PEG-DSPE and DSPE-PEG-NHS/(amine groups of PEI) at a molar ratio of 0.1 resulted in a higher uptake of CpGs into RAW264.7 cells, probably because of the presence of a hydrophobic lipid moiety. In addition, PEI-PEG-DSPE/CpG complexes triggered significant cytokine secretion (TNF-α) from RAW264.7 cells, comparable to that triggered by PEI/CpG complexes. Thus, PEI-PEG-DSPE conjugates could serve as biocompatible and efficient carriers of the immune stimulator CpG to the macrophages. |
| تدمد: | 1976-3816 1226-8372 |
| DOI: | 10.1007/s12257-020-0366-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e55dffd055913a4b402f22cca089dc99 https://doi.org/10.1007/s12257-020-0366-1 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........e55dffd055913a4b402f22cca089dc99 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19763816 12268372 |
|---|---|
| DOI: | 10.1007/s12257-020-0366-1 |