Characterisation of virus-related red tip disease in pineapple (Ananas comosus)
| العنوان: | Characterisation of virus-related red tip disease in pineapple (Ananas comosus) |
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| المؤلفون: | Kassim Faizah Abu, Ganesan Vadamalai, Khalid H. Alobaidi, Nor Azlina Hasbullah, Sarah Thaer, Jameel R. Al-Obaidi |
| المصدر: | Research Journal of Biotechnology. 16:10-17 |
| بيانات النشر: | World Researchers Associations, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Bioengineering, Applied Microbiology and Biotechnology, Biotechnology |
| الوصف: | Pineapple (Ananas comosus) is one of the tropical fruits that is cultivated in Malaysia for export as well as domestic use. Unfortunately, the production is heavily affected by the red tip disease which was detected in the southern part of Malaysia 3 decades ago. Despite the identification of this disease, causal agent receives less attention from researchers. Nevertheless, symptoms associated with this infection and the mode of transference inside the plantations indicate viral-like disease. RNA extracted from the pineapple infected leaves introduced to Nicotiana tabacum cv. Coker by kneading the plant leaves to extract fresh sap. Yellowish necrosis was detected three weeks after artificial inoculation. The staining of the fresh diseased sample and inoculated Nicotiana tabacum cv. Coker showed the existence of round particles with an average diameter of 94.25nm under the transmission electron microscope. SDS-PAGE analysis revealed proteins bands similar to those of tospovirus at 25, 55 and 128 kDa. Further protein identification and validation suggested for molecular confirmation of the disease causative agent. |
| تدمد: | 2278-4535 0973-6263 |
| DOI: | 10.25303/1612rjbt1017 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::e1c58e3e97895ca2c27d41c0d3bf44cb https://doi.org/10.25303/1612rjbt1017 |
| رقم الانضمام: | edsair.doi...........e1c58e3e97895ca2c27d41c0d3bf44cb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22784535 09736263 |
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| DOI: | 10.25303/1612rjbt1017 |