Rapid estimation of tumor cell percentage in brain tissue biopsy samples using inline cartridge extraction mass spectrometry
| العنوان: | Rapid estimation of tumor cell percentage in brain tissue biopsy samples using inline cartridge extraction mass spectrometry |
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| المؤلفون: | Eugene N. Nikolaev, Stanislav I. Pekov, Alexander Potapov, Igor Popov, V A Shurkhay, Denis S. Zavorotnyuk, D. S. Bormotov, V. A. Eliferov, Anatoly Sorokin, P.V. Nikitin |
| المصدر: | Analytical and Bioanalytical Chemistry. 413:2913-2922 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | medicine.diagnostic_test, Chemistry, 010401 analytical chemistry, Extraction (chemistry), Brain tumor, Tumor cells, 02 engineering and technology, 021001 nanoscience & nanotechnology, Mass spectrometry, medicine.disease, 01 natural sciences, Biochemistry, Mass spectrometry imaging, 0104 chemical sciences, Analytical Chemistry, Cartridge, Biopsy, medicine, 0210 nano-technology, Biomedical engineering, Ambient ionization |
| الوصف: | Tumor cell percentage (TCP) is an essential characteristic of biopsy samples that directly affects the sensitivity of molecular testing in clinical practice. Apart from clarifying diagnoses, rapid evaluation of TCP combined with various neuronavigation systems can be used to support decision making in neurosurgery. It is known that ambient mass spectrometry makes it possible to rapidly distinguish healthy from malignant tissues. In connection with this, here we demonstrate the possibility of using non-imaging ambient mass spectrometry to evaluate TCP in glial tumor tissues with a high degree of confidence. Molecular profiles of histologically annotated human glioblastoma tissue samples were obtained using the inline cartridge extraction ambient mass spectrometry approach. XGBoost regressors were trained to evaluate tumor cell percentage. Using cross-validation, it was estimated that the TCP was determined by the regressors with a precision of approximately 90% using only low-resolution data. This result demonstrates that ambient mass spectrometry provides an accurate method todetermine TCP in dissected tissues even without implementing mass spectrometry imaging. The application of such techniques offers the possibility to automate routine tissue screening and TCP evaluation to boost the throughput of pathology laboratories. |
| تدمد: | 1618-2650 1618-2642 |
| DOI: | 10.1007/s00216-021-03220-y |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::d484aaadade020f497aac1c383018403 https://doi.org/10.1007/s00216-021-03220-y |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........d484aaadade020f497aac1c383018403 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 16182650 16182642 |
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| DOI: | 10.1007/s00216-021-03220-y |