Lipid peroxidation and biochemical parameters in umbilical cord blood of well-adapted term newborns
| العنوان: | Lipid peroxidation and biochemical parameters in umbilical cord blood of well-adapted term newborns |
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| المؤلفون: | Branislav Liska, Želmíra Barošková, Gustáv Kováč, Jaroslav Hinšt, Denisa Maceková, Branislav Illek, Jana Perečková |
| المصدر: | Biologia. 64:409-413 |
| بيانات النشر: | Springer Science and Business Media LLC, 2009. |
| سنة النشر: | 2009 |
| مصطلحات موضوعية: | medicine.medical_specialty, Thiobarbituric acid, Cell Biology, Plant Science, Malondialdehyde, Phosphate, Biochemistry, Umbilical cord, Lipid peroxidation, chemistry.chemical_compound, Endocrinology, medicine.anatomical_structure, chemistry, Internal medicine, Blood plasma, Genetics, medicine, TBARS, Uric acid, Animal Science and Zoology, Molecular Biology, Ecology, Evolution, Behavior and Systematics |
| الوصف: | Lipid peroxidation (LPX) can play an important role in the development of pathological changes of foetal and neonatal tissues. We investigated LPX and biochemical parameters in plasma from mixed umbilical cord (m.u.c.) blood and acid-base balance (ABB) parameters in m.u.c. blood of well-adapted full-term newborns. LPX products were estimated as thiobarbituric acid reacting substances (TBARS) and were expressed by using of malondialdehyde (MDA) as a standard solution. Intensity of LPX was estimated in vitro in m.u.c. blood plasma without and with added LPX activator (125 μM L-ascorbate plus 5 μM FeSO4) and in the incubated plasma (30 min, 37°C) under both conditions. Actual TBARS (3.51 ± 0.49 nmol/mL) were determined in the non-incubated plasma without the added LPX activator. Approximately twice higher TBARS were found in the incubated plasma without the LPX activator (7.29 ± 2.17 nmol/mL) or with it (8.57 ± 2.20 nmol/mL), as well as in the non-incubated plasma after its addition (7.38 ± 1.98 nmol/mL). All analysed biochemical parameters (Fe, total iron-binding capacity, uric acid, proteins, Mg, Ca, phosphate, glucose, K, Na, Cl, ALT, AST, GMT, CK, LD, HBD, AMS, ALP, ACP) and ABB parameters were within their reference ranges. The actual TBARS levels were found being positively correlated with α-hydroxybutyrate dehydrogenase (HBD) activity and negatively with pO2. These results suggest that LPX in m.u.c. blood plasma might be activated. This activation could probably depend on extent of hypoxia. TBARS and their formation in vitro could be suitable parameters of LPX in m.u.c. blood. |
| تدمد: | 1336-9563 0006-3088 |
| DOI: | 10.2478/s11756-009-0055-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::d2e1bce8c783ee57f304499d28a0dc60 https://doi.org/10.2478/s11756-009-0055-1 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........d2e1bce8c783ee57f304499d28a0dc60 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 13369563 00063088 |
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| DOI: | 10.2478/s11756-009-0055-1 |