Bioremediation is a technique that uses microorganisms to reduce the con centration and/or the toxicidade of the pollutants, so it can be considered as an environmentally promising alternative to clean oil spill shorelines. The main objective of this work was to analyze the behavior of laboratory tests on bioremediation , with NPK nutrient to stimulate biodegradation of oil spills. The bioremediation process was monitored by geochemical analysis of the extracted oil from the sediments. The seawater used on the process was analyzed for nutrients con tents (phosphate and ammonium ions) and also for toxicity (Microtox tests). All results show that the NKP fertilizer can be used on bioremediation on sites affected by oil spill.