Protective Mechanism of Common Buckwheat (Fagopyrum esculentum Moench.) against Nonalcoholic Fatty Liver Disease Associated with Dyslipidemia in Mice Fed a High-Fat and High-Cholesterol Diet
| العنوان: | Protective Mechanism of Common Buckwheat (Fagopyrum esculentum Moench.) against Nonalcoholic Fatty Liver Disease Associated with Dyslipidemia in Mice Fed a High-Fat and High-Cholesterol Diet |
|---|---|
| المؤلفون: | Xu-Cong Lv, Li Ni, Pingfan Rao, Wei-Dong Bai, Zi-Rui Huang, Qiu-Yi Li, Jia-Cong Deng, Yi-Chen Lin, Ying-Jia Cao, Bin Liu |
| المصدر: | Journal of Agricultural and Food Chemistry. 68:6530-6543 |
| بيانات النشر: | American Chemical Society (ACS), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0106 biological sciences, medicine.medical_specialty, Bile acid, medicine.drug_class, 010401 analytical chemistry, nutritional and metabolic diseases, Lipid metabolism, General Chemistry, Metabolism, Pentose phosphate pathway, Biology, medicine.disease, 01 natural sciences, 0104 chemical sciences, Excretion, Endocrinology, Internal medicine, Nonalcoholic fatty liver disease, Hyperlipidemia, medicine, General Agricultural and Biological Sciences, Dyslipidemia, 010606 plant biology & botany |
| الوصف: | This study aimed to investigate the protective mechanism of common buckwheat (Fagopyrum esculentum Moench.) against nonalcoholic fatty liver disease (NAFLD) associated with dyslipidemia in mice that were fed a high-fat and high-cholesterol diet (HFD). Results showed that oral supplementation of common buckwheat significantly improved physiological indexes and biochemical parameters related to dyslipidemia and NAFLD in mice fed with HFD. Furthermore, the HFD-induced reductions in fecal short-chain fatty acids were reversed by common buckwheat intervention, which also increased the fecal bile acid (BA) abundance compared with HFD-induced hyperlipidemic mice. Liver metabolomics based on ultraperformance liquid chromatography-quadrupole time-of-flight mass spectrometry demonstrated that common buckwheat supplementation made significant regulatory effects on the pentose phosphate pathway, starch and sucrose metabolism, primary BA biosynthesis, and so forth. The results of high-throughput sequencing revealed that common buckwheat supplementation significantly altered the structure of the intestinal microbiota in mice fed with HFD. The correlations between lipid metabolic parameters and intestinal microbial phylotypes were also revealed by the heatmap and network. Additionally, common buckwheat intervention regulated the mRNA expressions of genes responsible for liver lipid metabolism and BA homeostasis, thus promoting BA synthesis and excretion. These findings confirmed that common buckwheat has the outstanding ability of improving lipid metabolism and could be used as a potential functional food for the prevention of NAFLD and hyperlipidemia. |
| تدمد: | 1520-5118 0021-8561 |
| DOI: | 10.1021/acs.jafc.9b08211 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c5868917f95cb91a3971d821ce12c58f https://doi.org/10.1021/acs.jafc.9b08211 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........c5868917f95cb91a3971d821ce12c58f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15205118 00218561 |
|---|---|
| DOI: | 10.1021/acs.jafc.9b08211 |