Structure and function of a nitrifying biofilm as determined by microelectrodes and fluorescent oligonucleotide probes
| العنوان: | Structure and function of a nitrifying biofilm as determined by microelectrodes and fluorescent oligonucleotide probes |
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| المؤلفون: | R. I. Amann, N. P. Revsbech, L. H. Larsen, A. Schramm |
| المصدر: | Water Science and Technology. 36:263-270 |
| بيانات النشر: | IWA Publishing, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Environmental Engineering, Water Science and Technology |
| الوصف: | Microelectrodes for O2 and NO2−/NO3− and fluorescently labelled 16S rRNA-targeted oligonucleotide probes were combined to examine the activity and stratification of nitrifying bacteria in a trickling filter biofilm. Microprofiles showed that O2 consumption and NO3−/NO2− production were restricted to the upper 50-100 μm of the biofilm. The vertical distribution of the nitrifying bacteria Nitrosomonas sp. and Nitrobacter sp. was investigated by fluorescent in situ hybridisation (FISH) with specific oligonucleotides. Nitrifiers formed a dense layer of cells and cell clusters in the upper part of the biofilm. This correlates well with the measured activity profiles. Ammonia- and nitrite-oxidisers occurred in close vicinity to each other supporting a fast sequential metabolism from ammonia to nitrate. Both species were not restricted to the oxic part of the biofilm, but also appeared -in lower numbers- in the anoxic layers on the bottom of the biofilm. A short term decrease in the O2 concentration of the bulk water resulted in a quick decrease in O2 penetration and metabolic rates inside the biofilm. However, neither the stratification nor the cellular ribosome content of nitrifiers changed within a few hours. |
| تدمد: | 1996-9732 0273-1223 |
| DOI: | 10.2166/wst.1997.0062 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c5343c45fc84fce7a0757d4088130622 https://doi.org/10.2166/wst.1997.0062 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........c5343c45fc84fce7a0757d4088130622 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19969732 02731223 |
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| DOI: | 10.2166/wst.1997.0062 |