In this paper, performance characteristics of cycles were studied when mixed working fluid was used for ocean thermal energy conversion (OTEC). Among the various mixed refrigerants for industrial heat-pump, R32/R152a used in ocean thermal energy conversion system. For simulations, R32/R152a were used in exist-ing closed cycle and Kalina cycle which is used only ammonia and water as mixed refrigerant. Temperature of the warm heat source was 26 and 29 celsius degree, temperature of the cold heat source was 5 celsius degree. In results of simulation, Gross power of the closed cycle on R32 was 22kW, and efficiency of the cycle was 2.02%. When the mixed refrigerant of R32/R152a, in the ratio of 90 to 10, gross power of the closed cycle was 29.93kW, and efficiency of the cycle was 2.78%. Gross power and cycle efficiency of R32/R152a increased by 36% and 37% than those of existing single refrigerant. Additionally, the same simu-lations were conducted in Kalina cycle with the same various composition ratio of mixed refrigerant.