Advancing HPLC-PDA-HRMS-SPE-NMR Analysis of Coumarins in Coleonema album by Use of Orthogonal Reversed-Phase C18 and Pentafluorophenyl Separations
| العنوان: | Advancing HPLC-PDA-HRMS-SPE-NMR Analysis of Coumarins in Coleonema album by Use of Orthogonal Reversed-Phase C18 and Pentafluorophenyl Separations |
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| المؤلفون: | Johannes Van Staden, Rita de Cássia Lemos Lima, Simone M. Gramsbergen, Kenneth T. Kongstad, Dan Staerk, Anna K. Jäger |
| المصدر: | Journal of Natural Products. 80:1020-1027 |
| بيانات النشر: | American Chemical Society (ACS), 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Pharmacology, Chromatography, 010405 organic chemistry, Chemistry, Organic Chemistry, Extraction (chemistry), Ethyl acetate, Pharmaceutical Science, Nuclear magnetic resonance spectroscopy, Fractionation, Mass spectrometry, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, 010404 medicinal & biomolecular chemistry, chemistry.chemical_compound, Complementary and alternative medicine, Phase (matter), Coleonema album, Drug Discovery, Molecular Medicine, Hplc pda |
| الوصف: | A hyphenated procedure involving high-performance liquid chromatography, photodiode array detection, high-resolution mass spectrometry, solid-phase extraction, and nuclear magnetic resonance spectroscopy, i.e., HPLC-PDA-HRMS-SPE-NMR, has proven an effective technique for the identification of compounds in complex matrices. Most HPLC-PDA-HRMS-SPE-NMR investigations reported so far have relied on analytical-scale reversed-phase C18 columns for separation. Herein is reported the use of an analytical-scale pentafluorophenyl column as an orthogonal separation method following fractionation of a crude ethyl acetate extract of leaves of Coleonema album on a preparative-scale C18 column. This setup allowed the HPLC-PDA-HRMS-SPE-NMR analysis of 23 coumarins, including six new compounds, 8-O-β-d-glucopyranosyloxy-6-(2,3-dihydroxy-3-methylbut-1-yl)-7-methoxycoumarin (4), (Z)-6-(4-β-d-glucopyranosyloxy-3-methylbut-2-en-1-yl)-7-hydroxycoumarin (6), 6-(4-β-d-glucopyranosyloxy-3-methylbut-1-yl)-7-hydroxycoumarin (8), (Z... |
| تدمد: | 1520-6025 0163-3864 |
| DOI: | 10.1021/acs.jnatprod.6b01020 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c31e6cf1624df7eb9815eded5d72c466 https://doi.org/10.1021/acs.jnatprod.6b01020 |
| رقم الانضمام: | edsair.doi...........c31e6cf1624df7eb9815eded5d72c466 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15206025 01633864 |
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| DOI: | 10.1021/acs.jnatprod.6b01020 |