Induction of triggering receptor expressed on myeloid cells (TREM-1) in airway epithelial cells by 1,25(OH)2 vitamin D3
| العنوان: | Induction of triggering receptor expressed on myeloid cells (TREM-1) in airway epithelial cells by 1,25(OH)2 vitamin D3 |
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| المؤلفون: | Sunghan Yim, Gill Diamond, Puneet Dhawan, Lisa K. Ryan, Laura W. McMahon, Isaura Rigo, Sylvia Christakos |
| المصدر: | Innate Immunity. 18:250-257 |
| بيانات النشر: | SAGE Publications, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Innate immune system, medicine.medical_treatment, Immunology, Antimicrobial peptides, Cell Biology, respiratory system, Biology, Microbiology, Molecular biology, Cathelicidin, Infectious Diseases, Beta defensin, Cell culture, Vitamin D Response Element, medicine, Respiratory epithelium, Receptor, Molecular Biology |
| الوصف: | The airway epithelium plays a role in host defense through the binding of innate immune receptors, which leads to the activation of inflammatory mediators, including antimicrobial peptides. The active form of vitamin D, 1,25(OH)2D3, induces the expression of the antimicrobial peptide LL-37 in both myeloid cells and airway epithelial cells (AEC). Here, we demonstrate that mRNA encoding triggering receptor expressed on myeloid cells (TREM)-1 was induced up to 12-fold by 1,25(OH)2D3 in normal human bronchial epithelial (NHBE) cells and in well-differentiated cultures of six airway epithelial cell lines from patients with cystic fibrosis and healthy individuals. TREM-2 and DAP12 were also expressed in airway cultures, but not induced by vitamin D. Induction occurs through a vitamin D response element identified in its proximal promoter region, and was regulated by PU.1 expressed in the AEC. Activation of TREM-1 by a cross-linking antibody led to an induction of both human β-defensin-2 and TNF-α mRNA, demonstrating its functionality in these cells. Our results expand on the role played by the airway epithelium in innate immunity and suggest that vitamin D can modulate the innate immune defense of the airway epithelium, and could potentially be developed as an adjunctive therapy for airway infections. |
| تدمد: | 1753-4267 1753-4259 |
| DOI: | 10.1177/1753425911399796 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::c182bb1bb517ff2b983b20567dd2da61 https://doi.org/10.1177/1753425911399796 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........c182bb1bb517ff2b983b20567dd2da61 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17534267 17534259 |
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| DOI: | 10.1177/1753425911399796 |