Mucin induces CRISPR-Cas defence in an opportunistic pathogen

التفاصيل البيبلوغرافية
العنوان: Mucin induces CRISPR-Cas defence in an opportunistic pathogen
المؤلفون: Janne J. Ravantti, Noora Rantanen, Gabriel Magno de Freitas Almeida, Lotta-Riina Sundberg, Ville Hoikkala
بيانات النشر: Cold Spring Harbor Laboratory, 2021.
سنة النشر: 2021
مصطلحات موضوعية: 0303 health sciences, Innate immune system, biology, 030306 microbiology, Mucin, Virulence, biology.organism_classification, Microbiology, 03 medical and health sciences, Immune system, Flavobacterium columnare, CRISPR, Pathogen, Bacteria, 030304 developmental biology
الوصف: Parasitism by bacteriophages has led to the evolution of a variety of defense mechanisms in their host bacteria. However, it is unclear what factors lead to specific defenses being deployed upon phage infection. To explore this question, we exposed the bacterial fish pathogenFlavobacterium columnareto its virulent phage V156 in the presence of a eukaryotic host signal (mucin). All tested conditions led to some level of innate immunity, but the presence of mucin led to a dramatic increase in CRISPR spacer acquisition, especially in low nutrient conditions where over 60% of colonies had obtained at least one new spacer. Additionally, we show that the presence of a competitor bacterium further increases CRISPR spacer acquisition inF. columnare. These results suggest that ecological factors are important in determining defense strategies against phages, and that the concentration of phages on metazoan surfaces may select for the diversification of bacterial immune systems.
DOI: 10.1101/2021.08.10.455787
URL الوصول: https://explore.openaire.eu/search/publication?articleId=doi_________::c07a3788adc64ae832a87d8ac61e6d9a
https://doi.org/10.1101/2021.08.10.455787
Rights: OPEN
رقم الانضمام: edsair.doi...........c07a3788adc64ae832a87d8ac61e6d9a
قاعدة البيانات: OpenAIRE
الوصف
DOI:10.1101/2021.08.10.455787