التفاصيل البيبلوغرافية
| العنوان: |
Soybean seed coat chitinase as a defense protein against the stored product pestCallosobruchus maculatus |
| المؤلفون: |
Jamile G Conceição, Valdirene Moreira Gomes, Jonas Perales, Kayan Eudorico Ventury, Andre Ts Ferreira, Leonardo Fr De Sá, José Xavier-Filho, Eduardo Ag Oliveira, Antonia Ea Oliveira, Izabela Silva dos Santos, Nadia C. M. Silva, Kátia Vs Fernandes, Monique Costa |
| المصدر: |
Pest Management Science. 74:1449-1456 |
| بيانات النشر: |
Wiley, 2018. |
| سنة النشر: |
2018 |
| مصطلحات موضوعية: |
0106 biological sciences, 0301 basic medicine, Cuticle, media_common.quotation_subject, Insect, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Chitin, Plant defense against herbivory, media_common, Larva, biology, fungi, food and beverages, General Medicine, biology.organism_classification, Callosobruchus maculatus, 010602 entomology, 030104 developmental biology, Biochemistry, chemistry, Insect Science, Chitinase, biology.protein, PEST analysis, Agronomy and Crop Science |
| الوصف: |
BACKGROUND Chitinases (EC 3.2.1.14) are enzymes involved in the breaking of the β-1,4-glycosidic linkages of chitin. In insects, chitin is present mainly in the cuticle and in peritrophic membranes and peritrophic gel. Enzymes with the potential to damage peritrophic membranes and gel, such as chitinase, have been associated with plant defense systems. Identification and characterization of seed coat chitinase as a plant defense molecule may indicate a more effective target for manipulation strategies, which may lead to the prevention of consumption of embryonic tissues by larvae and consequently minimization of seed damage. RESULTS We studied the efficiency of soybean seed coat chitinase as a defense molecule against the insect Callosobruchus maculatus. The seed coat chitinase was isolated and identified by mass spectrometry, immunoreacted with an anti-chitinase antibody and shown to have activity against chitin azure and 4-methylumbelliferyl β-D-N,N',N''-triacetylchitotrioside. A chitinase fraction incorporated in artificial cotyledons at 0.1% reduced larval survival by approximately 77%, and at 0.5%, the reduction in larval mass was 60%. Fluorescein isothiocyanate (FITC)-labeled chitinase was detected in the guts and feces of larvae. At 25% in thick artificial seed coats, chitinase showed a high toxicity to larvae, with mortality of 90% and a reduction of larval mass of 87%. CONCLUSION Seed coat chitinase is an important seed defense molecule not only in the cotyledons but also in seed coats, acting as part of the array of defense mechanisms against Callosobruchus maculatus. © 2017 Society of Chemical Industry. |
| تدمد: |
1526-498X |
| DOI: |
10.1002/ps.4832 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::bd6e8805dd443f52d25e3009803aa74a
https://doi.org/10.1002/ps.4832 |
| Rights: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........bd6e8805dd443f52d25e3009803aa74a |
| قاعدة البيانات: |
OpenAIRE |