In the present work, Zn-incorporated TiO2 coatings are prepared through a one-step micro-arc oxidation (MAO) method on a grade 4 pure titanium with the addition of either Na2Zn-EDTA solution or ZnO nanoparticles (NPs) as Zn sources. The microstructural features of both Zn-incorporated TiO2 coatings were systematically examined. It is revealed that different Zn sources result in significant difference of phase component, chemical state, composition and morphology between the resultant Zn-incorporated MAO coatings. Zn species could be present as ZnO and Zn(OH)2 in the coating when Na2Zn-EDTA was used as Zn source whereas the presence of ZnO nano-clusters is obvious on the coating surface with ZnO NPs as Zn source. The addition of ZnO NPs during the MAO process also leads to a lower Zn content of the resultant coating, which is more defective with increased thickness in comparison to that of Na2Zn-EDTA. Further, antibacterial property and osteogenic activity of both Zn-incorporated coatings were examined. Both Zn-incorporated coatings exhibit favourable bacterial inhibition ability and bone formability, suggesting the successful synthesis of bifunctional coatings through the facile one-step micro-arc oxidation method.