Simplified Method for Purification of Mouse β1H
| العنوان: | Simplified Method for Purification of Mouse β1H |
|---|---|
| المؤلفون: | Toshio Kaidoh, Shunnosuke Natsuume-Sakai, Teizo Fujita, Morinobu Takahashi, Yuko Takata |
| المصدر: | Complement. 1:44-51 |
| بيانات النشر: | S. Karger AG, 1984. |
| سنة النشر: | 1984 |
| مصطلحات موضوعية: | Gel electrophoresis, Chromatography, medicine.diagnostic_test, biology, Chemistry, Immunology, Size-exclusion chromatography, Hematology, Immunoelectrophoresis, Monospecific antibody, Complement system, Sepharose, Affinity chromatography, medicine, biology.protein, Beta (finance) |
| الوصف: | A simple three-step method was described for purification of murine beta 1H, one of the essential regulatory proteins of complement system. The method consists of heparin-Sepharose affinity chromatography; gel filtration on a Sepharose 6B column, and DNA-cellulose affinity chromatography. By this method over 10 mg of beta 1H can be purified by more than 200-fold from 100-ml of EDTA serum of various strains. Overall yield of beta 1H was about 45%. The purified beta 1H was homogeneous as judged by SDS-polyacrylamide gel electrophoresis and immunoelectrophoresis. The purified mouse beta 1H showed physicochemical properties very similar to those described for human beta 1H: mouse beta 1H is a beta-globulin consisting of a single polypeptide chain of molecular weight of 160,000. Purified mouse beta 1H retained its functional activity as the essential cofactor for the cleavage of fluid-phase human C3b by the human C3b inactivator. Immunization of rabbits with the purified mouse beta 1H resulted in the production of the potent and monospecific antibody. |
| تدمد: | 2504-2424 0253-5076 |
| DOI: | 10.1159/000467813 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b912e0800d9eaeb3a38239b8f8fe48dd https://doi.org/10.1159/000467813 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........b912e0800d9eaeb3a38239b8f8fe48dd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 25042424 02535076 |
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| DOI: | 10.1159/000467813 |