Gastrin peptide isolation in broiler chicken
| العنوان: | Gastrin peptide isolation in broiler chicken |
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| المؤلفون: | Apratim Maity, R. K. Das, G. R.Sahoo, A. K. Mandal |
| المصدر: | Indian Journal of Animal Research. |
| بيانات النشر: | Agricultural Research Communication Center, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | chemistry.chemical_classification, medicine.medical_specialty, Chromatography, General Veterinary, Molecular mass, digestive, oral, and skin physiology, Aqueous two-phase system, Broiler, Peptide, Biology, digestive system, Endocrinology, chemistry, Distilled water, Internal medicine, medicine, Animal Science and Zoology, Centrifugation, Gizzard, Gastrin |
| الوصف: | The present study was undertaken to isolate gastrin peptide from the antral tissue of broiler chicken. The chicken antrums, i.e. tissue pieces from a narrow zone at gizzard – duodenal junction were collected, boiled in distilled water, followed by centrifugation at 0° C. The supernatant was collected, added to isopropanol and stirred overnight. After addition of dichloromethane, the aqueous phase was partitioned, aspirated and lyophilized. The electrophoretic analysis (SDS-PAGE) of the antral sample was carried out after running it along with a reference protein marker. Characterization of the antral extract revealed a total of eleven peptide bands having relative molecular weights (Mr) ranging from 4.6 to114.5 kDa, out of which peptides having Mr of 22.6 and 26.3 kDa were major ones. The protein or peptide band showing the lowest relative molecular weight (Mr, 4.6 kDa) was identified as the gastrin. |
| تدمد: | 0976-0555 0367-6722 |
| DOI: | 10.18805/ijar.6694 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b76b72afe5df88ce96363fda4c3f3384 https://doi.org/10.18805/ijar.6694 |
| رقم الانضمام: | edsair.doi...........b76b72afe5df88ce96363fda4c3f3384 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 09760555 03676722 |
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| DOI: | 10.18805/ijar.6694 |