Cytoplasmic ?-catenin in esophageal cancers
| العنوان: | Cytoplasmic ?-catenin in esophageal cancers |
|---|---|
| المؤلفون: | Kenshu Kawanishi, Masatoshi Inoue, Makoto Yamamoto, Yutaka Kimura, Morito Monden, Yuichiro Doki, Takehiro Utsunomiya, Nariaki Fukuchi, Hitoshi Shiozaki, Toshimasa Tsujinaka |
| المصدر: | International Journal of Cancer. 84:174-178 |
| بيانات النشر: | Wiley, 1999. |
| سنة النشر: | 1999 |
| مصطلحات موضوعية: | Cancer Research, Pathology, medicine.medical_specialty, Cell, Gene mutation, Biology, Molecular biology, Epithelium, Cell membrane, medicine.anatomical_structure, Oncology, Epidermoid carcinoma, Cytoplasm, Catenin, medicine, Cell adhesion |
| الوصف: | beta-Catenin has 2 distinct roles in E-cadherin-mediated cell adhesion and carcinogenesis through APC gene mutation. One occurs at cell-adhesion sites, where cadherins become linked to the actin-based cytoskeleton. The others occur in the cytoplasm and nuclei and are thought to regulate cell transformation. We studied these different beta-catenins and evaluated their significance in carcinogenesis. Fresh surgical specimens were obtained from 22 patients with squamous-cell carcinoma of the esophagus. beta-Catenin in the free soluble fraction and the insoluble fraction was immunoblotted separately. At the same time, its localization was observed by immuno-histochemical techniques. In the normal esophageal epithelium, 91% of beta-catenin was detected in the insoluble fraction and beta-catenin staining occurred at the cell membrane, in co-existence with E-cadherin. In cancerous tissues, the amount of soluble beta-catenin was significantly (about 4-fold) higher than in normal tissues. Also, in cancerous tissues with higher amounts of soluble beta-catenin, immuno-histochemical techniques revealed the presence of beta-catenin in the cytoplasm and nuclei, as well as in the cell membrane. However, in samples with lower amounts of beta-catenin, expression was found only at the cell boundaries. The amount of soluble beta-catenin was not associated with the clinico-pathological grading of the tumors. Our results show that the accumulation of free soluble beta-catenin in the cytoplasm and nuclei frequently occurs during carcinogenesis of the squamous epithelium of the esophagus. |
| تدمد: | 1097-0215 0020-7136 |
| DOI: | 10.1002/(sici)1097-0215(19990420)84:2<174::aid-ijc14>3.0.co;2-e |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b67bb3ca078bb2c55820ba70bc0123bb https://doi.org/10.1002/(sici)1097-0215(19990420)84:2<174::aid-ijc14>3.0.co;2-e |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........b67bb3ca078bb2c55820ba70bc0123bb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
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| DOI: | 10.1002/(sici)1097-0215(19990420)84:2<174::aid-ijc14>3.0.co;2-e |