Amniotic membrane allografts maintain key biological properties post SCCO2 and lyophilization processing
| العنوان: | Amniotic membrane allografts maintain key biological properties post SCCO2 and lyophilization processing |
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| المؤلفون: | Jennifer S. McDaniel, Anthony J. Johnson, Jennifer L Wehmeyer, Lauren E. Cornell, David O. Zamora |
| المصدر: | Journal of Biomaterials Applications. 35:592-601 |
| بيانات النشر: | SAGE Publications, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0303 health sciences, Biocompatibility, Chemistry, Biomedical Engineering, Cold storage, Sterilization (microbiology), Cryopreservation, Biomaterials, Extracellular matrix, 03 medical and health sciences, 0302 clinical medicine, Membrane, Biological property, 030221 ophthalmology & optometry, Wound healing, 030304 developmental biology, Biomedical engineering |
| الوصف: | Amniotic membrane (AM) has been shown to enhance corneal wound healing due to the abundance of growth factors, cytokines, and extracellular matrix (ECM) proteins inherent to the tissue. As such, AM has garnered widespread clinical utility as a biological dressing for a number of ophthalmic and soft tissue applications. The preparation, sterilization, and storage procedures used to manufacture AM grafts are extremely important for the conservation of inherent biological components within the membrane. Current processing techniques use harsh chemicals and sterilization agents that can compromise the fundamental wound healing properties of AM. Furthermore, commercially available cryopreserved AM products require specific storage conditions (e.g., ultra-low freezers) thereby limiting their clinical availability in austere environments. Supercritical carbon dioxide (SCCO2) technology allows for the sterilization of biological tissues without the resulting degradation of integral ECM proteins and other factors often seen with current tissue sterilization processes. With this study we demonstrate that lyophilized AM, sterilized using SCCO2, maintains similar biochemical properties and biocompatibility as that of commercially available AM products requiring specialized cold storage conditions. |
| تدمد: | 1530-8022 0885-3282 |
| DOI: | 10.1177/0885328220952585 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b3af082f29545732226cc1f59a3cf16a https://doi.org/10.1177/0885328220952585 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........b3af082f29545732226cc1f59a3cf16a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15308022 08853282 |
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| DOI: | 10.1177/0885328220952585 |