Investigation of the role of ATPase Inhibitory Factor 1 in pulmonary arterial hypertension
| العنوان: | Investigation of the role of ATPase Inhibitory Factor 1 in pulmonary arterial hypertension |
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| المؤلفون: | Zachary Brown, Robert Batori, Hunter Sellers, Stephen Haigh, Zsuzsanna Bordan, Madison West, Scott Barman, David Fulton |
| المصدر: | Physiology. 38 |
| بيانات النشر: | American Physiological Society, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Physiology |
| الوصف: | Pulmonary arterial hypertension is a rare and debilitating condition with a five-year survival rate of only 34% without treatment. The origins of pulmonary arterial hypertension are complex and incompletely understood and elevated pulmonary artery pressures result from excessive vascular remodeling due to functional changes in endothelial, vascular smooth muscle cells (VSMCs), and adventitial cells. Recent papers have explored the importance of metabolic switching of VSMCs in pulmonary hypertension in the transition towards a proliferative, apoptotic resistant phenotype which involves a shift from fatty acid oxidation to increased glycolysis. We hypothesized that ATPase Inhibitory Factor 1 (ATPIF1) in VSMCs could function as a metabolic switch that promotes a shift from fatty acid oxidation to glycolysis to increase the severity of pulmonary arterial hypertension. This hypothesis will be investigated using Western blot, Seahorse extracellular flux assays, qRT-PCR, and imaging of GFP-labelled ATPIF1. In preliminary data, we have cloned ATPIF1 and transfected cells to show overexpression of ATPIF1 decreases the maximal respiratory rate while increasing extracellular acidification, a measure of glycolysis. Future goals are to investigate the links between hypoxia, hypoxia-inducible factors and ATPIF1, the role of increased glycolysis in pulmonary smooth muscle cells, and ultimately whether ATPIF1 impacts of the development of pulmonary arterial hypertension in mice. This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process. |
| تدمد: | 1548-9221 1548-9213 |
| DOI: | 10.1152/physiol.2023.38.s1.5733452 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::b01a478c947f6c4dbedca87c4b82e032 https://doi.org/10.1152/physiol.2023.38.s1.5733452 |
| رقم الانضمام: | edsair.doi...........b01a478c947f6c4dbedca87c4b82e032 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15489221 15489213 |
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| DOI: | 10.1152/physiol.2023.38.s1.5733452 |