Fut2 −/− (Non-Secretor) Mice Exhibit High Vwf Levels
| العنوان: | Fut2 −/− (Non-Secretor) Mice Exhibit High Vwf Levels |
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| المؤلفون: | Jill M. Johnsen, Ermias Yohannes, Thomas W Walsh |
| المصدر: | Blood. 120:2184-2184 |
| بيانات النشر: | American Society of Hematology, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | chemistry.chemical_classification, Fucosyltransferase, biology, Chemistry, Immunology, Cell Biology, Hematology, H antigen, Biochemistry, Molecular biology, fluids and secretions, Antigen, Von Willebrand factor, Polyclonal antibodies, hemic and lymphatic diseases, ABO blood group system, biology.protein, Antibody, Glycoprotein |
| الوصف: | Abstract 2184 Levels of the critical adhesive glycoprotein von Willebrand Factor (VWF) are widely variable in human populations. Two-thirds of VWF variation is heritable, of which one-third is due to the carbohydrate blood group ABO. Another closely related carbohydrate blood group, Secretor (FUT2), has also been reported to influence VWF levels, but this association is controversial. In humans, FUT2 nonsense mutations are common and result in the loss of FUT2 enzyme activity and the absence of Lewis and ABH sugars in mucosal secretions, hence the designation of FUT2 null individuals as “non-Secretors”. The FUT2 (Secretor) fucosyltransferase makes H antigen (fucalpha1à2betagal) in mucosal tissues, which can subsequently be fucosylated by other Lewis enzymes (FUT3, FUT6, etc.) to generate the variations within the Lewis blood groups system, or serve as a substrate for the ABO glycosyltransferase to generate ABH structures. To investigate the hypothesis that Secretor influences VWF, we examined Vwf levels in Fut2 knock-out (non-Secretor) mice. The Fut2 knock-out mouse line backcrossed >20 generations to C57BL6/J was recovered from cryopreserved stock at the Jackson Laboratory. Eight to 12 week old male mice were selected for study to avoid the potential effects of estrous and aging on Vwf levels. Platelet-poor plasma was obtained from Fut2−/− (non-Secretor, n=7) and their Fut2+/+ (Secretor, n=5) littermates and assayed for Vwf antigen (Vwf:Ag) by sandwich ELISA using polyclonal anti-VWF antibodies [rabbit anti-human VWF (Dako); sheep anti-human VWF (Abcam)] and a pooled C57BL6/J plasma standard. Non-Secretor (Fut2−/−) animals exhibited Vwf:Ag levels nearly double those of their Secretor (Fut2+/+) littermates (p Vwf:Ag (+/− 1SD) Secretor (Fut2+/+) 103 +/− 39 Non-Secretor (Fut2−/−) 196 +/− 65 p=0.01, Student's unpaired two-tailed ttest. Disclosures: No relevant conflicts of interest to declare. |
| تدمد: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v120.21.2184.2184 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::aa211c0596403acd2120f85972fd0b5c https://doi.org/10.1182/blood.v120.21.2184.2184 |
| رقم الانضمام: | edsair.doi...........aa211c0596403acd2120f85972fd0b5c |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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| DOI: | 10.1182/blood.v120.21.2184.2184 |