Cloning of Rabbit TR4 and Its Bone Cell-Specific Activity to Suppress Estrogen Receptor-Mediated Transactivation1
| العنوان: | Cloning of Rabbit TR4 and Its Bone Cell-Specific Activity to Suppress Estrogen Receptor-Mediated Transactivation1 |
|---|---|
| المؤلفون: | Shoichi Masushige, Yoshihiko Koga, Chikara Honda, Yoshikazu Kuboi, Masashi Nakatsuka, Shigeaki Kato, Rika Miki, Hideyuki Harada |
| المصدر: | Endocrinology. 139:204-212 |
| بيانات النشر: | The Endocrine Society, 1998. |
| سنة النشر: | 1998 |
| مصطلحات موضوعية: | Messenger RNA, Exon, Transactivation, Endocrinology, Nuclear receptor, Complementary DNA, Bone cell, RNA splicing, Biology, Receptor, Molecular biology |
| الوصف: | To clone a new nuclear receptor, we screened a rabbit heart complementary DNA (cDNA) library with degenerate oligonucleotide probes corresponding to the DNA-binding domain of nuclear receptors, which is highly conserved among receptors. One of the cDNA clones, clone 23, encodes a novel protein of 596 amino acids, and predicted molecular mass is 66 kDa. Homology search analysis identified this protein as rabbit TR4 (TR4–0). We also cloned the cDNA encoding a rabbit TR4 isoform (TR4–1), which lacks the putative C-terminal ligand-binding domain (350 amino acids) caused by a 23-bp exon deletion, which probably occurred during messenger RNA (mRNA) splicing. Northern blot analysis showed that TR4s are expressed with two kinds of mRNAs (9.0 kb and 2.8 kb), both of which are relatively abundant in brain, testis, and bone. RT-PCR analysis, using pairs of primers specific for each TR4, showed that both types of receptor express in various tissues. Furthermore, both are present in primary osteoblasts and bone marrow... |
| تدمد: | 1945-7170 0013-7227 |
| DOI: | 10.1210/endo.139.1.5682 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a85ffedbd286efecc033aba7442e818b https://doi.org/10.1210/endo.139.1.5682 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........a85ffedbd286efecc033aba7442e818b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19457170 00137227 |
|---|---|
| DOI: | 10.1210/endo.139.1.5682 |