Abstract 5514: Antitumor activity of (R,R’)-4′-methoxy-1-naphthylfenoterol in a rat C6 glioma xenograft model in the mouse
| العنوان: | Abstract 5514: Antitumor activity of (R,R’)-4′-methoxy-1-naphthylfenoterol in a rat C6 glioma xenograft model in the mouse |
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| المؤلفون: | Kevin G. Becker, Jean-François Cloix, Yongqing Zhang, Irving W. Wainer, Rajib K. Paul, Anuradha Ramamoorthy, Arnaud Paris, Michel Bernier |
| المصدر: | Cancer Research. 73:5514-5514 |
| بيانات النشر: | American Association for Cancer Research (AACR), 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | Antitumor activity, Cancer Research, Chemistry, 4'-methoxy-1-naphthylfenoterol, Cancer, Body weight, medicine.disease, Molecular biology, C6 glioma, Oncology, In vivo, In vitro proliferation, medicine, Cancer cell lines |
| الوصف: | (R,R’)-4’-methoxy-1-naphthylfenoterol (MNF) inhibits in vitro proliferation of several types of cancer cell lines. In this study, the in vivo antitumor effects of MNF were evaluated using rat C6 glioma cells implanted subcutaneously into the lower flank of 5 week-old NMRI/Nude female Swiss mice (0.5 x 10(6) cells, 100μl). Three days after the inoculation, the mice were subjected to intraperitoneal injections of saline or MNF (10μl/g, 2mg/kg of body weight) for 5 days per week for 2 weeks. Tumor volumes were measured everyday using slide calipers. Significant reductions in mean tumor volumes were observed in mice receiving MNF when compared with the saline-treated group (p < 0.001, n=17-19). At the end of the study, animals were sacrificed and tumors were collected for cDNA microarray, quantitative PCR and immunoblot analyses. Comparison of cDNA microarray analysis of tumors from mice treated with MNF vs. saline demonstrated a significant decrease in expression of genes involved in cellular proliferation, including mitotic checkpoint kinase MAD3L (BUB1B), cyclin-dependent kinase inhibitor 3 (CDKN3) and cyclin A2 (CCNA2), as well as molecular markers for glioblastoma, such as oligodendrocyte transcription factor 1 (OLIG1) and SRY-box 4 (SOX4). Target gene validation is currently in progress. Our results show that MNF reduces growth of flank xenografted C6 glioma tumors and expression of genes related to tumor proliferation and suggest that this compound may be useful in cancer treatment. This work was supported in part by the Intramural Research Program of the NIA/ NIH. Citation Format: Rajib K. Paul, Anuradha Ramamoorthy, Yongqing Zhang, Kevin G. Becker, Arnaud Paris, Jean-Francois Cloix, Michel Bernier, Irving W. Wainer. Antitumor activity of (R,R’)-4′-methoxy-1-naphthylfenoterol in a rat C6 glioma xenograft model in the mouse. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 5514. doi:10.1158/1538-7445.AM2013-5514 |
| تدمد: | 1538-7445 0008-5472 |
| DOI: | 10.1158/1538-7445.am2013-5514 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a7718bb443974e325630025e60d7defb https://doi.org/10.1158/1538-7445.am2013-5514 |
| رقم الانضمام: | edsair.doi...........a7718bb443974e325630025e60d7defb |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15387445 00085472 |
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| DOI: | 10.1158/1538-7445.am2013-5514 |