Alterations of the Purinergic Regulation in Mesenteric Arteries of Pannexin-1-Knockout Mice
| العنوان: | Alterations of the Purinergic Regulation in Mesenteric Arteries of Pannexin-1-Knockout Mice |
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| المؤلفون: | Yuri V. Panchin, Valery I. Shestopalov, Dina K Gaynullina, O. O. Kiryukhina, Olga S. Tarasova |
| المصدر: | Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology. 12:62-69 |
| بيانات النشر: | Pleiades Publishing Ltd, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | 0301 basic medicine, Agonist, medicine.medical_specialty, Electrical impedance myography, medicine.drug_class, Purinergic receptor, Biophysics, Cell Biology, Smooth muscle contraction, Pannexin, Biochemistry, 03 medical and health sciences, Thromboxane A2, chemistry.chemical_compound, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, chemistry, Internal medicine, medicine, Mesenteric arteries, Phenylephrine, medicine.drug |
| الوصف: | Pannexin 1 (Panx1) forms plasma membrane channels that release ATP, an important vascular tone regulator. However, despite the abundant expression of Panx1 in the vascular system, its effects on smooth muscle contraction are not evident in all arteries. In this study, we tested the hypothesis that the functional consequences of Panx1 deficiency can be masked by the augmented action of ATP secreted in a Panx1-independent way. Experiments were performed on small mesenteric arteries obtained from Panx1-knockout (Panx1–/–) and C57Bl/6 (Panx1+/+) male mice using wire myography of endothelium-denuded arterial preparations and reverse-transcription quantitative PCR techniques. Arterial contractile responses to phenylephrine did not differ in two experimental groups. Ecto-ATPase inhibitor ARL67156 (100 μM) potentiated the responses to phenylephrine in Panx1+/+ but not in Panx1–/–, while ARL67156 did not affect the contractile responses to the thromboxane A2 receptor agonist in any of the two groups. Contractile responses to exogenous ATP (10 μM) were smaller in Panx1+/+ than in Panx1–/– mice. By contrast, NTPDase1 mRNA content was higher in Panx1+/+ than in Panx1–/– mice. These results suggest that ATP released from smooth muscle cells through Panx1 channels can potentiate contractile responses of murine mesenteric arteries upon activation of α1-adrenoceptors. In Panx1–/– mice an increased arterial ATP sensitivity and diminished NTPDase1 activity may augment the contractile effects of Panx1-independent ATP. |
| تدمد: | 1990-7494 1990-7478 |
| DOI: | 10.1134/s1990747818010075 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::a439d92b4d38d13598b052f779d6c8b3 https://doi.org/10.1134/s1990747818010075 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........a439d92b4d38d13598b052f779d6c8b3 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 19907494 19907478 |
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| DOI: | 10.1134/s1990747818010075 |