Top2A FISH with cytokeratin discriminators in breast cancer using automated high throughput analysis
| العنوان: | Top2A FISH with cytokeratin discriminators in breast cancer using automated high throughput analysis |
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| المؤلفون: | Jiri Bartek, Heli Nevanlinna, Carl Blomqvist, William E. Pierceall, David T. Weaver, Päivi Heikkilä, K. M. Sprott, Tuomas Heikkinen |
| المصدر: | Journal of Clinical Oncology. 28:e11061-e11061 |
| بيانات النشر: | American Society of Clinical Oncology (ASCO), 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Oncology, Cancer Research, medicine.medical_specialty, Chemotherapy, Pathology, Tissue microarray, Anthracycline, business.industry, medicine.medical_treatment, medicine.disease, Radiation therapy, Cytokeratin, Breast cancer, Internal medicine, medicine, Immunohistochemistry, Personalized medicine, business |
| الوصف: | e11061 Background: Biomarker profiling increasingly provides key information for discriminating good versus poor outcome in chemotherapy treatment decisions (personalized medicine). For breast cancer patients treated with anthracycline-based regimens, tumor TOP2A expression levels examined by IHC are not as predictive of overall survival as TOP2A gene copy alterations. The SWOG S9313/Int0137 study report suggests that TOP2A status is not predictive of recurrence with anthracycline-based regimens. We examined TOP2A by a FISH-based approach in a large breast cancer study and stratified patients for analysis by therapy (anthracycline-based with radiation therapy (RT), nonanthracycline -based with RT or RT alone). Methods: FFPE specimens were configured on Tissue Microarrays (TMAs) for efficient analysis. 1,491 breast cancer patient tumors (4 cores/patient; 0.6 mm diameter, 5 um thickness) were evaluated for TOP2A status by FISH coupled with cytokeratin (CK, clone AE1/AE3) immunofluorescence (IF). All slides ... |
| تدمد: | 1527-7755 0732-183X |
| DOI: | 10.1200/jco.2010.28.15_suppl.e11061 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::9d6e90874cde14be4d494467572dc283 https://doi.org/10.1200/jco.2010.28.15_suppl.e11061 |
| رقم الانضمام: | edsair.doi...........9d6e90874cde14be4d494467572dc283 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15277755 0732183X |
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| DOI: | 10.1200/jco.2010.28.15_suppl.e11061 |