Enzyme-linked immunosorbent assay to quantitate serum ferritin in the northern fur seal (Callorhinus ursinus)
| العنوان: | Enzyme-linked immunosorbent assay to quantitate serum ferritin in the northern fur seal (Callorhinus ursinus) |
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| المؤلفون: | Lisa M. Mazzaro, Patricia S. Chavey, David J. St. Aubin, Gordon A. Andrews, J. Lawrence Dunn |
| المصدر: | Zoo Biology. 23:79-84 |
| بيانات النشر: | Wiley, 2004. |
| سنة النشر: | 2004 |
| مصطلحات موضوعية: | Hemolytic anemia, CATS, biology, medicine.diagnostic_test, General Medicine, medicine.disease, biology.organism_classification, Ferritin, Andrology, Hypoproteinemia, Callorhinus ursinus, Immunology, biology.protein, medicine, Serum iron, Animal Science and Zoology, Fur seal, Hemochromatosis |
| الوصف: | Serum ferritin concentration correlates with tissue iron stores in humans, horses, calves, dogs, cats, and pigs. Serum ferritin is considered the best serum analyte to predict total body iron stores in these species, and is more reliable than serum iron or total iron-binding capacity, both of which may be affected by disorders unrelated to iron adequacy or excess (including hypoproteinemia, chronic infection, hemolytic anemia, hypothyroidism, renal disease, and drug administration). Iron overload has been documented to result in hemochromatosis in captive northern fur seals (Callorhinus ursinus); therefore, we developed an enzyme-linked immunosorbent assay (ELISA) to measure serum ferritin in this species. The assay uses two murine anti-canine ferritin monoclonal antibodies in a sandwich arrangement that was originally used in an ELISA to measure serum ferritin in dogs. Ferritin isolated from fur seal liver was used as a standard. Ferritin standards were linear from 0 to 50 ng/ml. Recovery of purified ferritin from fur seal serum varied from 89% to 99%. The within-assay variability was 6%, and the assay-to-assay variability for two different samples was 10% and 16%. Zoo Biol 23:79-84, 2004.© 2004 Wiley-Liss, Inc. |
| تدمد: | 1098-2361 0733-3188 |
| DOI: | 10.1002/zoo.10125 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::9c358b4cd89f7a69b819f98b64e6fb5a https://doi.org/10.1002/zoo.10125 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........9c358b4cd89f7a69b819f98b64e6fb5a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10982361 07333188 |
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| DOI: | 10.1002/zoo.10125 |