Detection of Epstein-Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV-infected rhesus monkeys byin situ hybridization
| العنوان: | Detection of Epstein-Barr virus small RNAs EBER1 and EBER2 in lymphomas of SIV-infected rhesus monkeys byin situ hybridization |
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| المؤلفون: | Walter Bodemer, Sabine Pingel, Franz-Josef Kaup, Gerhard Hunsmann, Kerstin Mätz-Rensing, Horst Hannig |
| المصدر: | International Journal of Cancer. 72:160-165 |
| بيانات النشر: | Wiley, 1997. |
| سنة النشر: | 1997 |
| مصطلحات موضوعية: | Cancer Research, viruses, animal diseases, virus diseases, In situ hybridization, Biology, Simian immunodeficiency virus, medicine.disease, medicine.disease_cause, biology.organism_classification, Virology, Epstein–Barr virus, Virus, Herpesviridae, Lymphoma, Oncology, immune system diseases, hemic and lymphatic diseases, Immunology, medicine, Gammaherpesvirinae, Immunodeficiency |
| الوصف: | SIV infection of macaques is a well-established animal model for studying the pathogenesis of HIV infection in humans. During the course of SIV infection, up to 40% of cynomolgus macaques (Macaca fascicularis) develop SIV-associated non-Hodgkin's lymphomas. In the present study, we characterized malignant lymphomas of SIV mac 251/132H-infected rhesus macaques (Macaca mulatta) of our cohort in terms of clinical outcome, histopathology and EBV association. Histopathologic changes of lymphoid malignancies were classified according to the Kiel classification. For detection of the EBV-encoded small RNAs EBERI and EBER2, a method of non-isotopic in situ hybridization was established. The presence of EBNA-2 antigens was assessed by immunohisto-chemistry. Seven of 43 rhesus macaques developed highly malignant B-cell lymphomas of the centroblastic, immunoblastic and Burkitt subtypes within 18-29 months post-experimental SIV infection. In situ hybridization revealed the presence of small EBERI and -2 RNAs in 6 of 7 disease cases. EBNA-2 antigens could be demonstrated in only 4 of 7 tissue specimens. As expected, the Burkitt-type of lymphoma was negative for EBNA-2 antigen staining. In accordance with findings on SIV-associated lymphomas of cynomolgus macaques, infection with an EBV-related herpesvirus could be demonstrated in almost 90% of lymphomas in SIV-infected rhesus macaques. In contrast, the presence of EBV in lymphomas had been documented previously in only 30-40% of HIV-infected patients. Further studies should thus define the precise role of herpesvirus infection for lymphomagenesis in SIV- and HIV-induced immunodeficiency. |
| تدمد: | 1097-0215 0020-7136 |
| DOI: | 10.1002/(sici)1097-0215(19970703)72:1<160::aid-ijc23>3.0.co;2-m |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8faf464520f1ccae1cc43dd185bc935a https://doi.org/10.1002/(sici)1097-0215(19970703)72:1<160::aid-ijc23>3.0.co;2-m |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........8faf464520f1ccae1cc43dd185bc935a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 10970215 00207136 |
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| DOI: | 10.1002/(sici)1097-0215(19970703)72:1<160::aid-ijc23>3.0.co;2-m |