Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation
| العنوان: | Foxl2 of the Hong Kong catfish (Clarias fuscus): cDNA cloning, tissue distribution and changes in gene expression towards methyltestosterone, estradiol and letrozole exposure of the fries during gonadal differentiation |
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| المؤلفون: | Chunhua Zhu, Si-Ping Deng, Tian-li Wu, Huapu Chen, Shang-li Shi, Guangli Li, Wen-da Wang, Jing Sun |
| المصدر: | Genes & Genomics. 37:669-677 |
| بيانات النشر: | Springer Science and Business Media LLC, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | endocrine system, medicine.medical_specialty, Aromatase inhibitor, biology, medicine.drug_class, Letrozole, Period (gene), Biochemistry, Sexual dimorphism, Endocrinology, Hypothalamus, Internal medicine, Gene expression, Genetics, medicine, biology.protein, Methyltestosterone, Aromatase, Molecular Biology, medicine.drug |
| الوصف: | Winged helix/forkhead transcription factor gene 2 (Foxl2) plays a crucial role during early ovarian development in fish. Sex steroids and aromatase inhibitors can regulate gonadal differentiation in teleosts. To address the role of Foxl2 in gonadal differentiation, foxl2 was isolated and characterized from the Hong Kong catfish, Clarias fuscus. Tissue distribution analysis by quantitative real-time PCR (qPCR) showed that the foxl2 mRNA was highest in the ovaries; moderate in the female pituitary and hypothalamus; but weak in the forebrain, liver, testis and male pituitary, with a sexually dimorphic pattern in which there was higher expression overall in females versus males. Treatment of the fries during the period of gonadal differentiation [2–30 days post hatching (dph)] with 17α-methyltestosterone (MT), an aromatase inhibitor (letrozole) and 17β-estradiol (E2) affected the expression of foxl2. The expression of foxl2 was highest before gonadal differentiation (12 dph), but decreased significantly after gonadal differentiation (18-30 dph). In addition, MT and letrozole were able to down-regulate foxl2, but E2 up-regulated foxl2 during gonadal morphological differentiation. These results indicate the significant roles of Foxl2 in the gonadal differentiation of C. fuscus. |
| تدمد: | 2092-9293 1976-9571 |
| DOI: | 10.1007/s13258-015-0296-z |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8b2f8f25cdec53c496f3912dfcee2bed https://doi.org/10.1007/s13258-015-0296-z |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........8b2f8f25cdec53c496f3912dfcee2bed |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 20929293 19769571 |
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| DOI: | 10.1007/s13258-015-0296-z |