التفاصيل البيبلوغرافية
| العنوان: |
Differential Expression and Regulation of Angiopoietin-3 in Mouse Uterus during Preimplantation Period |
| المؤلفون: |
Shi-Jie Li, Zhan-Peng Yue, Bin Guo, Dang-Dang Li, Xue-Chao Tian, Shou-Tang Wang, Juxiong Liu, Xueming Zhang |
| المصدر: |
Journal of Experimental Zoology Part B: Molecular and Developmental Evolution. 318:316-324 |
| بيانات النشر: |
Wiley, 2012. |
| سنة النشر: |
2012 |
| مصطلحات موضوعية: |
medicine.medical_specialty, Pregnancy, medicine.drug_class, Decidua, Uterus, Decidualization, In situ hybridization, Biology, medicine.disease, Reverse transcription polymerase chain reaction, medicine.anatomical_structure, Endocrinology, Estrogen, Internal medicine, Genetics, medicine, Ovariectomized rat, Molecular Medicine, Animal Science and Zoology, hormones, hormone substitutes, and hormone antagonists, Ecology, Evolution, Behavior and Systematics, Developmental Biology |
| الوصف: |
Angiogenesis is necessary for successful implantation and decidualization. This study was to investigate the differential expression of angiopoietin-3 (Ang-3) in mouse uterus during early pregnancy and its regulation by steroid hormones using in situ hybridization and reverse transcription polymerase chain reaction (RT-PCR). There was no detectable Ang-3 mRNA signal on days 1–5 of pregnancy by in situ hybridization. On day 6 of pregnancy, a low level of Ang-3 mRNA signal was seen in the primary decidua. Ang-3 mRNA expression gradually increased on days 7 and 8 of pregnancy along with the development of decidua, and its expression scope was also expanded. The RT-PCR result indicated that Ang-3 mRNA expression was low on days 1–4 of pregnancy. On day 5, as embryo implanted, Ang-3 mRNA was highly expressed in mouse uterus, and the expression gradually increased on days 6–8 of pregnancy, with peak level on day 8 of pregnancy. Similarly, Ang-3 mRNA was also strongly expressed in decidualized cells under artificial decidualization. Compared with the delayed uterus, a high level of Ang-3 mRNA expression was detected in activated implantation uterus by RT-PCR. In the ovariectomized mouse uterus, Ang-3 mRNA expression increased and reached the highest level at 12 hr after injection of estrogen, progesterone, and estrogen plus progesterone, respectively. These results suggest that Ang-3 may play an important role during the process of mouse decidualization. Both estrogen and progesterone can induce the expression of Ang-3 in ovariectomized mouse uterus. J. Exp. Zool. (Mol. Dev. Evol.) 318:316–324, 2012. © 2012 Wiley Periodicals, Inc. |
| تدمد: |
1552-5007 |
| DOI: |
10.1002/jez.b.22449 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::8998c25927e870a09f9ea6f0bfc1ad88
https://doi.org/10.1002/jez.b.22449 |
| Rights: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........8998c25927e870a09f9ea6f0bfc1ad88 |
| قاعدة البيانات: |
OpenAIRE |