Immune fibroblasts are unconventional antigen presenting cells and nonprofessional phagocytes in Lyme arthritis
| العنوان: | Immune fibroblasts are unconventional antigen presenting cells and nonprofessional phagocytes in Lyme arthritis |
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| المؤلفون: | Robert B Lochhead, Rebecca Danner, Amanda Wahhab, Joseph Rouse |
| المصدر: | The Journal of Immunology. 204:148.29-148.29 |
| بيانات النشر: | The American Association of Immunologists, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Synovial fibroblasts are the primary cell type in synovial tissue and are one of the first cell types involved in immune responses to Borrelia burgdorferi invasion of joint tissue. Recent studies of human Lyme arthritis have shown that synovial fibroblasts up-regulate numerous genes involved in T cell activation upon exposure to the Th1 cytokine IFN-gamma. However, animal model studies are required to better understand their role in host defense and pathogenesis in Lyme arthritis. Using primary synovial fibroblasts isolated from the tibiotarsal joint of C57BL/6 mice, we have found that fibroblasts stimulated with IFN-gamma and B. burgdorferi express MHC class II molecules, a number of costimulatory molecules, and effector cytokines involved in lymphocyte activation, including activation of CD4+ T cells and NK cells. MHC class II molecules isolated from stimulated fibroblasts contained MHC-II peptides, indicating that these cells are capable of presenting antigen to CD4+ T cells. Stimulation with IFN-gamma also reduced fibroblast migration, indicating these cells have impaired ability to repair damaged tissue. Synovial fibroblasts were also able to internalize B. burgdorferi in cell culture, indicating that these cells are capable of acting as nonprofessional phagocytes. Ongoing experiments will determine which lymphocyte subsets interact with inflammatory synovial fibroblasts, and whether these subsets are protective against B. burgdorferi invasion of joint tissue and/or arthritogenic. Together, these data indicate that immune fibroblasts are important in shaping tissue-specific immune responses to B. burgdorferi infection and subsequent inflammation. |
| تدمد: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.204.supp.148.29 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8647eef9332f50f193814026e5ad2066 https://doi.org/10.4049/jimmunol.204.supp.148.29 |
| رقم الانضمام: | edsair.doi...........8647eef9332f50f193814026e5ad2066 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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| DOI: | 10.4049/jimmunol.204.supp.148.29 |