Efficacy of long-term coral tissue storage in ethanol for genotyping studies
| العنوان: | Efficacy of long-term coral tissue storage in ethanol for genotyping studies |
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| المؤلفون: | M. J. H. van Oppen, A. R. Brown, Ray Berkelmans, Jason Doyle, E. F. Asbridge |
| المصدر: | Coral Reefs. 33:89-96 |
| بيانات النشر: | Springer Science and Business Media LLC, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | geography, geography.geographical_feature_category, biology, Tissue Preservation, Ecology, Coral, Coral reef, Aquatic Science, biology.organism_classification, DNA extraction, chemistry.chemical_compound, Symbiodinium, chemistry, Acropora, Food science, Genotyping, DNA |
| الوصف: | With climate change threatening the future of coral reefs, there is an urgent need for effective coral tissue preservation and repositories from which DNA can be extracted. Most collections use 95 % ethanol as the storage medium, but its efficacy for long-term storage for short-fragment DNA use remains poorly documented. We conducted an accelerated DNA aging trial on three species of coral to ascertain whether ethanol-stored tissue and skeleton samples could yield fit-for-purpose DNA at time scales of 100+ yrs. We conclude that even using a crude DNA extraction technique, samples kept at 40 °C for 20 months yielded DNA of sufficient quality for Symbiodinium and coral host genotyping. If stored at −20 °C, these samples are likely to still yield useable DNA after 100 yrs. Ethanol-stored samples compared favorably in terms of DNA quality, quantity and sample integrity with those stored in an analogue of the commercial storage buffer RNAlater ®. |
| تدمد: | 1432-0975 0722-4028 |
| DOI: | 10.1007/s00338-013-1106-1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::85d25ebaad6fe1b8306a536e98a52af8 https://doi.org/10.1007/s00338-013-1106-1 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........85d25ebaad6fe1b8306a536e98a52af8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14320975 07224028 |
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| DOI: | 10.1007/s00338-013-1106-1 |