Anti-platelet and anti-inflammatory properties of an ethanol-water red grape pomace extract
| العنوان: | Anti-platelet and anti-inflammatory properties of an ethanol-water red grape pomace extract |
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| المؤلفون: | Nikolaos S. Thomaidis, Elizabeth Fragopoulou, Smaragdi Antonopoulou, Maria Choleva, Maria Tsota, Anthi Panara, Vassiliki Boulougouri |
| المصدر: | Proceedings of the Nutrition Society. 79 |
| بيانات النشر: | Cambridge University Press (CUP), 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | Nutrition and Dietetics, medicine.drug_class, Linolenic acid, Pomace, Medicine (miscellaneous), Catechin, Anti-inflammatory, chemistry.chemical_compound, chemistry, medicine, Food science, Gallic acid, Quercetin, IC50, EC50 |
| الوصف: | Previous reports support that wine contains a mixture of micro-constituents in a proper quality and quantity that possess cardio-protective effect partly through Platelet-Activating Factor (PAF) inhibition. Grape pomace (GP) is a source of wine-like micro-constituents that may be a suitable alternative in food fortification. Limited data exist concerning their effects on thrombosis and inflammation. Therefore, the purpose of this study was to examine a grape pomace extract regarding its anti-platelet and anti-inflammatory properties. GP from four red grape varieties were extracted with 80% ethanol (GP:solvent 1:5w/v). The extract's total phenolic compounds were evaluated and the phenolic profile was performed by Ultrahigh-Performance-Liquid-Chromatography coupled to Mass-Spectrometry and the determination of fatty acids profile was performed by Gas-Chromatography. The extract's anti-platelet properties were tested in healthy volunteers’ platelet rich plasma by the light transmittance method, against three agonists: PAF, ADP and TRAP. The results expressed as IC50 values (μg of extract that cause 50% inhibition of aggregation) and EC50 values (agonist concentration that causes 50% of the maximum aggregation) in the extract's presence and absence. Concerning the extract's anti-inflammatory properties, peripheral blood mononuclear cells from healthy volunteers were pre-incubated with different extract concentrations, which were tested for their effect on cell viability, for 1 h and then stimulated with LPS for 4 h. Secretion of IL-1β and TNF-a was measured and normalized with the total cell protein. Phenolic compounds were calculated at 8.79 ± 1.17 mg gallic acid per g of GP. The most abundant ones were catechin, epicatechin and quercetin at 202.9 ± 6.9, 84.8 ± 1.5 and 83.7 ± 3.5μg per g of GP respectively. Out of the 18 fatty acids detected, the most abundant ones were palmitic, oleic, linoleic, and linolenic acid at 28.7 ± 0.1, 11.4 ± 0.01, 32.5 ± 0.07, 12.7 ± 0.005 g per 100 g of fat. The extract's IC50 was calculated at 162.1 ± 66.9, 181.2 ± 82.3 and 156.3 ± 97.5μg against PAF, ADP and TRAP, respectively. The EC50 values in the presence of 150μg extract were increased (lower platelet aggregation sensitivity) approximately at 100%, 45% and 13% against PAF, ADP and TRAP respectively, compared to EC50 values in the absence of extract. The presence of 500 and 1000μg/mL of extract reduced LPS-induced TNF-a secretion at approximately 38.2% (p = 0.04) and 6.0% (p < 0.000), respectively. Potent anti-platelet and anti-inflammatory properties are combined in a grape pomace extract. The use of its bioactive micro-constituents is likely to lead to the production of functional foods with cardioprotective properties. |
| تدمد: | 1475-2719 0029-6651 |
| DOI: | 10.1017/s0029665120003183 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::8225cf94bb66da357059dca9bdf7cc4f https://doi.org/10.1017/s0029665120003183 |
| Rights: | OPEN |
| رقم الانضمام: | edsair.doi...........8225cf94bb66da357059dca9bdf7cc4f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752719 00296651 |
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| DOI: | 10.1017/s0029665120003183 |