Do T cells from HIV-infected individuals have normal metabolic function?
| العنوان: | Do T cells from HIV-infected individuals have normal metabolic function? |
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| المؤلفون: | Genevieve Tyndale Clutton, Olivia Council, Yinyan Xu, Joanna Warren, De’Ashia Lee, Maria Abad Fernandez, Nancie Archin, JoAnn Kuruc, Joseph Eron, Cindy Gay, David Margolis, Nilu Goonetilleke |
| المصدر: | The Journal of Immunology. 198:125.2-125.2 |
| بيانات النشر: | The American Association of Immunologists, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | Immunology, Immunology and Allergy |
| الوصف: | Untreated HIV infection is characterized by generalized immune activation, T cell dysfunction, and ultimately the exhaustion of HIV-specific CD8 T cell responses. Durable virus suppression resulting from anti-retroviral therapy (ART) is associated with greatly improved immune function, but some phenotypic and functional abnormalities persist even after years of suppressive ART. We have observed that, relative to HIV-seronegative individuals, CD8 T cells from HIV+ participants on ART are skewed toward a more effector-like (T-bethigh Eomeslow) phenotype and have significantly reduced proliferative capacity in response to antigenic stimulation. Metabolic pathways play a key role in shaping T cell maturation, phenotype, and function, and the ability to produce ATP via both glycolytic and oxidative phosphorylation (mitochondrial) pathways is critical to T cell function and longevity. However, whether underlying metabolic abnormalities contribute to residual CD8 T cell dysfunction in HIV+ individuals on ART has not been fully explored. Here we compare the metabolic phenotype of T cells from HIV+ participants on ART with those from HIV-seronegative participants. We are using MitoTracker® dyes to assess mitochondrial mass and polarization in T cell memory subsets, ex vivo and in response to antigen, and measuring expression of the glucose transporter GLUT1 and the mitochondrial biogenesis master regulator PGC-1α in different CD8 T cell effector subsets. Our findings will inform whether phenotypic and functional abnormalities in CD8 T cells from HIV+ individuals on ART reflect underlying metabolic dysfunction and if metabolic interventions could improve T cell function. |
| تدمد: | 1550-6606 0022-1767 |
| DOI: | 10.4049/jimmunol.198.supp.125.2 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7d9d980cd7d56e006a9740bdeef5f8f7 https://doi.org/10.4049/jimmunol.198.supp.125.2 |
| رقم الانضمام: | edsair.doi...........7d9d980cd7d56e006a9740bdeef5f8f7 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15506606 00221767 |
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| DOI: | 10.4049/jimmunol.198.supp.125.2 |