التفاصيل البيبلوغرافية
| العنوان: |
Distinguishing modes of cell death using the ImageStream® multispectral imaging flow cytometer |
| المؤلفون: |
William E. Ortyn, David J. Perry, Cathleen A. Zimmerman, David Basiji, Thaddeus C. George, Brian E. Hall, Philip J. Morrissey, David H. Lynch, Michael J. Seo |
| المصدر: |
Cytometry Part A. :237-245 |
| بيانات النشر: |
Wiley, 2004. |
| سنة النشر: |
2004 |
| مصطلحات موضوعية: |
education.field_of_study, Programmed cell death, Histology, medicine.diagnostic_test, Population, Cell, Multispectral image, Cell Biology, Biology, Jurkat cells, Pathology and Forensic Medicine, Cell biology, Flow cytometry, medicine.anatomical_structure, Apoptosis, Annexin, medicine, education |
| الوصف: |
Background Here we demonstrate the ability of the ImageStream® 100 Multispectral Imaging Cytometer to discriminate between live, necrotic, and early and late apoptotic cells, using unique combinations of photometric and morphometric features. Methods Live, necrotic, and early and late apoptotic cells were prepared and analyzed by immunofluorescence microscopy, conventional flow cytometry, and imaging flow cytometry, both as single populations and as a heterogeneous mixture of cells. Results Live (annexin V−, 7-AAD−) and early apoptotic (annexin V+, 7-AAD−) cells were readily identifiable using either conventional or ImageStream based flow cytometric methods. However, inspection of multispectral images of cells demonstrated that the annexin V+, 7-AAD+ population contained both necrotic and late-stage apoptotic cells. Although these cells could not be distinguished using conventional flow cytometric techniques, they were separable using unique combinations of photometric and morphometric measures available using ImageStream technologies. Conclusions Using multispectral imagery, morphologically distinct cell populations can be distinguished using features not available with conventional flow cytometers. In particular, the ability to couple morphometric with photometric measures makes it possible to distinguish live cells from cells in the early phases of apoptosis, as well as late apoptotic cells from necrotic cells. © 2004 Wiley-Liss, Inc. |
| تدمد: |
1552-4922 |
| DOI: |
10.1002/cyto.a.20048 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::7d62c0eafe0a1e1b894554aa6b83c0d2
https://doi.org/10.1002/cyto.a.20048 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi...........7d62c0eafe0a1e1b894554aa6b83c0d2 |
| قاعدة البيانات: |
OpenAIRE |