A Predictive Statistical Model To Identify Thalassemic Phenotype (β0 vs β+) in Heterozygous β-Thalassemia
| العنوان: | A Predictive Statistical Model To Identify Thalassemic Phenotype (β0 vs β+) in Heterozygous β-Thalassemia |
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| المؤلفون: | Silvia de la Iglesia, Jose Manuel Calvo-Villas, María Francisca Zapata, Paloma Ropero, Francisco Sicilia, Alejandro Mayosky |
| المصدر: | Blood. 110:3823-3823 |
| بيانات النشر: | American Society of Hematology, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | congenital, hereditary, and neonatal diseases and abnormalities, medicine.medical_specialty, medicine.diagnostic_test, Thalassemia, Immunology, Cell Biology, Hematology, Odds ratio, Biology, Stepwise regression, Logistic regression, medicine.disease, Biochemistry, Confidence interval, symbols.namesake, Endocrinology, hemic and lymphatic diseases, Internal medicine, symbols, medicine, Globin, Mean corpuscular volume, Fisher's exact test |
| الوصف: | β thalassemias are a heterogeneous group of genetic alterations characterized by a decreasing (β+) or eliminating (β0) expression of b globin gene. Previous studies have reported that mean corpuscular volume (MCV) and mean corpuscular haemoglobin (MCH) values would be useful to differentiate between β+ and β0 thalassemia phenotypes in heterozygous carriers. An accurate and valid risk model for belonging to each of these groups may be valuable for prioritizing identification of genetic alterations in some populations. The aim of this study was to develop a theoretic model that combine information from the haematological indices for phenotypic prediction (β+ vs. β0) in heterozygous β thalassemia. The study was conducted on 238 unrelated β thalassemia carriers. Hematological parameters were obtained using analyzer Coulter ®GEN-S™. HbA2 and HbF were analysed by high performance liquid chromatography. Molecular analysis for β globin and α-globin genes was performed by real-time PCR, direct sequencing techniques and standard PCR techniques. β-thalassemic subjects who also exhibited either αthalassemia or extra α globin genes were excluded. Statistical significance was calculated by using the χ2 or Fisher exact test for qualitative variables and the t test for continuous variables. A multivariate logistic regression model, with stepwise selection, was fitted from a case group of 64 β+thalassemia subjects and a reference group of 174 β0carriers. Odds ratios and their 95% confidence intervals (CIs) were computed. Statistical analysis was performed with the SPSS 12.0 statistical software package. The mean level of HbA2 (4.5 ± 0.7% vs. 5.1 ± 0.7%, [p 4 vs. HbA2< 4, MCH> 20.5 vs. MCH< 20.5 and HbF> 1.5 vs. HbF< 1.5. The adjusted model implies that with a HbA2< 4, the chance of having β+thalassemia increase by a factor 8.3 (CI 1.6–43.1) compared with a HbA2> 4. Analogously, at a MCH > 20.5, the chance of having β +thalassemia increase by a factor 9.4 (CI 4.3–20.2) compared with a MCH< 20.5. Regarding to HbF, it is 1.3 times more likely (CI 1.1–1.9) to be included in the β+thalassemia group with HbF< 1.5 than with HbF> 1.5. For this adjusted model, with a 26,8% heterozygous β+thalassemia prevalence, model test performance characteristics included: sensitivity 61.4% and specificity 89.1% (p=0.000). The logistic multivariate model to allow accurate prediction of the phenotype of β thalassemia trait (β+ vs β0) in the 84.1% of the carriers. The Hosmer-Lemeshow statistics indicated fine goodness of fit of the logistic regression equation (P=0.739). In summary, this logistic regression analysis using a combination of hematological indices such as MCH, HbA2 and HbF has showed an acceptable value in predicting phenotype of heterozygous b thalassemia (β+ vs β0) in a predominant Spanish population. The present model should be validated by independent data from different populations. |
| تدمد: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v110.11.3823.3823 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7c2bb0c6edfc28ea34d4de3cb7802442 https://doi.org/10.1182/blood.v110.11.3823.3823 |
| رقم الانضمام: | edsair.doi...........7c2bb0c6edfc28ea34d4de3cb7802442 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15280020 00064971 |
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| DOI: | 10.1182/blood.v110.11.3823.3823 |