Cross-Species Proteomic Comparison of Outer Membrane Vesicles and Membranes of Francisella tularensis subsp. tularensis versus subsp. holarctica
| العنوان: | Cross-Species Proteomic Comparison of Outer Membrane Vesicles and Membranes of Francisella tularensis subsp. tularensis versus subsp. holarctica |
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| المؤلفون: | Jiri Stulik, Jana Klimentova, Klara Kubelkova, Pavel Rehulka, Jan Bavlovic, Ivona Pavkova |
| المصدر: | Journal of Proteome Research. 20:1716-1732 |
| بيانات النشر: | American Chemical Society (ACS), 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | 0301 basic medicine, 030102 biochemistry & molecular biology, biology, Chemistry, Virulence, General Chemistry, biology.organism_classification, Biochemistry, Virulence factor, Microbiology, Lipid A, 03 medical and health sciences, 030104 developmental biology, Proteome, Cell envelope, Bacterial outer membrane, Francisella tularensis, Bacteria |
| الوصف: | Release of outer membrane vesicles (OMV) is an important phenomenon in Gram-negative bacteria playing multiple roles in their lifestyle, including in relation to virulence and host-pathogen interaction. Francisella tularensis, unlike other bacteria, releases unusually shaped, tubular OMV. We present a proteomic comparison of OMV and membrane fractions from two F. tularensis strains: moderately virulent subsp. holarctica strain FSC200 and highly virulent subsp. tularensis strain SchuS4. Proteomic comparison studies routinely evaluate samples from the same proteome, but sometimes we must compare samples from closely related organisms. This raises quantification issues. We propose a novel approach to cross-species proteomic comparison based on an intersection protein database from the individual single-species databases. This is less prone to quantification errors arising from differences in the sequences. Consecutively comparing subproteomes of OMV and membranes of the two strains allows distinguishing differences in relative protein amounts caused by global expression changes from those caused by preferential protein packing to OMV or membranes. Among the proteins most differently packed into OMV between the two strains, we detected proteins involved in biosynthesis and metabolism of bacterial envelope components like O-antigen, lipid A, phospholipids, and fatty acids, as well as some major structural outer membrane proteins. The data are available via ProteomeXchange with identifier PXD022406. |
| تدمد: | 1535-3907 1535-3893 |
| DOI: | 10.1021/acs.jproteome.0c00917 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::7b94247dce5b80fe66893e8065109719 https://doi.org/10.1021/acs.jproteome.0c00917 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........7b94247dce5b80fe66893e8065109719 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15353907 15353893 |
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| DOI: | 10.1021/acs.jproteome.0c00917 |