Synergistic effect of Camellia sinensis and Withania somnifera on hydrogen peroxide-induced oxidative stress: an in vitro study
| العنوان: | Synergistic effect of Camellia sinensis and Withania somnifera on hydrogen peroxide-induced oxidative stress: an in vitro study |
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| المؤلفون: | Arti Devi, Nancy George, Vikas Kumar, Zaved Ahmed Khan |
| المصدر: | Research Journal of Biotechnology. 18:9-17 |
| بيانات النشر: | World Researchers Associations, 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | Bioengineering, Applied Microbiology and Biotechnology, Biotechnology |
| الوصف: | Camellia sinensis (CS) and Withania somnifera (WS) are well-known to have antioxidant and anti-ageing effects. However, information on their synergistic antioxidant effect is still not known. In this study, we have compared the % cell viability of oxidative stress induced 3T3-L1 cell lines after treatment with CS, WS alone and in combination. The methanolic extracts of CS and WS were prepared by three cycles of maceration with 24 h duration each. The effect of CS and WS alone and in combination was determined under three different conditions viz. pre-treatment, co-treatment and post-treatment at 24hrs, 48hrs and 72hrs with the help of MTT assay. CS+WS were found to show synergistic effect under pre-treatment conditions with % cell viability of 95.39 ± 0.7% at an optimum concentration of WS+CS (4.87μg/ml+15.22μg/ml) after 72 hrs. The combination index was found to be less than 1 at 24 hrs (0.278), 48 hrs (0.172) and 72 hrs (0.126) for combination of CS+WS indicating a synergistic effect of plant combination. |
| تدمد: | 2278-4535 0973-6263 |
| DOI: | 10.25303/1803rjbt09017 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::79d4ee667b0c71dbf4108aabcddabfc6 https://doi.org/10.25303/1803rjbt09017 |
| رقم الانضمام: | edsair.doi...........79d4ee667b0c71dbf4108aabcddabfc6 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22784535 09736263 |
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| DOI: | 10.25303/1803rjbt09017 |