Cloning and expression analysis of porcine small adipocyte factor 1 (SMAF1) gene
| العنوان: | Cloning and expression analysis of porcine small adipocyte factor 1 (SMAF1) gene |
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| المؤلفون: | Zheng Rong, Jiang Si-Wen, Li Feng-E, Yang Hong-Wen |
| المصدر: | Chinese Journal of Agricultural Biotechnology. 4:151-155 |
| بيانات النشر: | Cambridge University Press (CUP), 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | Cloning, chemistry.chemical_classification, Biology, Molecular biology, Amino acid, Conserved sequence, chemistry.chemical_compound, chemistry, Adipocyte, Complementary DNA, Gene expression, Agronomy and Crop Science, Peptide sequence, Gene, Biotechnology |
| الوصف: | cDNA of the porcine small adipocyte factor 1 (SMAF1) gene was amplified by reverse transcriptase-polymerase chain reaction (RT-PCR) with degenerate primers designed according to the conserved sequences between human and mouse genes. The cDNA (GenBank accession No. DQ191892) containing a complete encoding region was 256 bp in length, sharing 86 and 78% identity with that of human and mouse, respectively. Comparison of the deduced amino acid sequence between porcine SMAF1 and the human, mouse, cattle and rat protein showed that the amino acid similarity was 81, 67, 84 and 70%, respectively. The results of semi-quantitative RT-PCR showed that the porcine gene was expressed abundantly in adipose tissue, and at a significantly lower level in lean-type pigs (Large White pigs) than in lard-type pigs (Meishan pigs) at the age of 4 months (PSMAF1 gene may have similar functions as in other species, that is, it may regulate adipogenesis and/or adipocyte function. |
| تدمد: | 1479-2370 1479-2362 |
| DOI: | 10.1017/s1479236207001507 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::77c6184a340475d506b691aae945566e https://doi.org/10.1017/s1479236207001507 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........77c6184a340475d506b691aae945566e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14792370 14792362 |
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| DOI: | 10.1017/s1479236207001507 |