Morphology, phylogeny and toxin analysis of Pseudo-nitzschia pseudodelicatissima (Bacillariophyceae) isolated from the Thermaikos Gulf, Greece
| العنوان: | Morphology, phylogeny and toxin analysis of Pseudo-nitzschia pseudodelicatissima (Bacillariophyceae) isolated from the Thermaikos Gulf, Greece |
|---|---|
| المؤلفون: | Panagiota Katikou, Georgios Nikolaidis, Dimitra Papaefthimiou, Alexandra Panou, Kimon K. Moschandreou, Eleni Kalopesa |
| المصدر: | Phycologia. 49:260-273 |
| بيانات النشر: | Informa UK Limited, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Species complex, Domoic acid, Plant Science, Aquatic Science, Biology, Ribosomal RNA, biology.organism_classification, chemistry.chemical_compound, Diatom, chemistry, Phylogenetics, Amnesic shellfish poisoning, Botany, Internal transcribed spacer, Pseudo-nitzschia |
| الوصف: | is a diatom species that has been associated with the production of the neurotoxin domoic acid (DA), causative agent of amnesic shellfish poisoning. However, the taxonomic revision of this species complex resulted in uncertain identity and, subsequently, toxicity of the strains tentatively identified under light microscope as P. pseudodelicatissima. Six strains isolated from the Thermaikos Gulf during the period from August to October 2008 were cultured and tested for production of DA. All strains were studied in morphological detail using electron microscopy and were identified as P. pseudodelicatissima. The identification was supported by phylogenetic analyses of the large subunit and internal transcribed spacer (ITS) ribosomal (r)DNA regions, along with the secondary structure of ITS-2 rDNA region. DA was detected in all cultures and in three of the strains in quantifiable amounts up to 3.08 ng ml 21 . Two of the strains were studied over the course of a batch culture growth experiment and DA was detected from the mid-exponential until late-stationary phases; the maximum whole-culture DA levels were measured in late-exponential to early-stationary phases. This study confirms the production of DA by P. pseudodelicatissima in culture. |
| تدمد: | 2330-2968 0031-8884 |
| DOI: | 10.2216/ph09-42.1 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::73c8540fdc00661c5e7c52060c7f68ea https://doi.org/10.2216/ph09-42.1 |
| رقم الانضمام: | edsair.doi...........73c8540fdc00661c5e7c52060c7f68ea |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 23302968 00318884 |
|---|---|
| DOI: | 10.2216/ph09-42.1 |