التفاصيل البيبلوغرافية
| العنوان: |
Cell number and chondrogenesis in human mesenchymal stem cell aggregates is affected by the sulfation level of heparin used as a cell coating |
| المؤلفون: |
Johnna S. Temenoff, Elda A Treviño, Jennifer Lei |
| المصدر: |
Journal of Biomedical Materials Research Part A. 104:1817-1829 |
| بيانات النشر: |
Wiley, 2016. |
| سنة النشر: |
2016 |
| مصطلحات موضوعية: |
0301 basic medicine, Materials science, Cell growth, Growth factor, medicine.medical_treatment, Cell, Mesenchymal stem cell, Metals and Alloys, Biomedical Engineering, 02 engineering and technology, Heparin, 021001 nanoscience & nanotechnology, Chondrogenesis, Cell biology, Biomaterials, Glycosaminoglycan, 03 medical and health sciences, 030104 developmental biology, medicine.anatomical_structure, Ceramics and Composites, medicine, 0210 nano-technology, Fibroblast, medicine.drug |
| الوصف: |
For particular cell-based therapies, it may be required to culture mesenchymal stem cell (MSC) aggregates with growth factors to promote cell proliferation and/or differentiation. Heparin, a negatively charged glycosaminoglycan (GAG) is known to play an important role in sequestration of positively charged growth factors and, when incorporated within cellular aggregates, could be used to promote local availability of growth factors. We have developed a heparin-based cell coating and we believe that the electrostatic interaction between native heparin and the positively charged growth factors will result in (1) higher cell number in response to fibroblast growth factor-2 (FGF-2) and 2) greater chondrogenic differentiation in response to transforming growth factor-β1 (TGF-β1), compared to a desulfated heparin coating. Results revealed that in the presence of FGF-2, by day 14, heparin-coated MSC aggregates increased in DNA content 8.5 ± 1.6 fold compared to day 1, which was greater than noncoated and desulfated heparin-coated aggregates. In contrast, when cultured in the presence of TGF-β1, by day 21, desulfated heparin-coated aggregates upregulated gene expression of collagen II by 86.5 ± 7.5 fold and collagen X by 37.1 ± 4.7 fold, which was higher than that recorded in the noncoated and heparin-coated aggregates. These observations indicate that this coating technology represents a versatile platform to design MSC culture systems with pairings of GAGs and growth factors that can be tailored to overcome specific challenges in scale-up and culture for MSC-based therapeutics. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1817-1829, 2016. |
| تدمد: |
1549-3296 |
| DOI: |
10.1002/jbm.a.35713 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::7209c9c9a3f25551d2965166ffa14f7c
https://doi.org/10.1002/jbm.a.35713 |
| Rights: |
OPEN |
| رقم الانضمام: |
edsair.doi...........7209c9c9a3f25551d2965166ffa14f7c |
| قاعدة البيانات: |
OpenAIRE |