التفاصيل البيبلوغرافية
| العنوان: |
Sesamol ameliorates radiation induced DNA damage in hematopoietic system of whole body γ-irradiated mice |
| المؤلفون: |
Nabo Kumar Chaudhury, Arun Kumar, J.S. Adhikari, Sandeep Choudhary |
| المصدر: |
Environmental and Molecular Mutagenesis. 59:79-90 |
| بيانات النشر: |
Wiley, 2017. |
| سنة النشر: |
2017 |
| مصطلحات موضوعية: |
0301 basic medicine, Epidemiology, Chemistry, DNA damage, Health, Toxicology and Mutagenesis, Amifostine, Pharmacology, Ionizing radiation, Comet assay, Toxicology, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, medicine.anatomical_structure, medicine, Bone marrow, Radiation Induced DNA Damage, Micronucleus, Sesamol, Genetics (clinical), medicine.drug |
| الوصف: |
Ionizing radiation exposure is harmful and at high doses can lead to acute hematopoietic radiation syndrome. Therefore, agents that can protect hematopoietic system are important for development of radioprotector. Sesamol is a potential molecule for development of radioprotector due to its strong free radical scavenging and antioxidant properties. In the present study, sesamol was evaluated for its role in DNA damage and repair in hematopoietic system of γ-irradiated CB57BL/6 mice and compared with amifostine. C57BL/6 male mice were administered with sesamol 20 mg/kg (i.p.) followed by 2 Gy whole body irradiation (WBI) at 30 min. Mice were sacrificed at 0.5, 3, 24 h postirradiation; bone marrow, splenocytes, and peripheral blood lymphocytes were isolated to measure DNA damages and repair using alkaline comet,γ-H2AXand micronucleus assays. An increase in % of tail DNA was observed in all organs of WBI mice. Whereas in pre-administered sesamol reduced %DNA in tail (P ≤ 0.05). Sesamol has also reduced formation of radiation induced γ-H2AX foci after 0.5 h in these organs and further lowered to respective control values at 24 h of WBI. Similar reduction of % DNA in tail and γ-H2AX foci were observed with amifostine (P ≤ 0.05). Analysis of mnPCE frequency at 24 h has revealed similar extent of protection by sesamol and amifostine. Interestingly, both sesamol and amifostine, alone and with radiation, also increased the granulocytes count significantly compared to the control (P ≤ 0.05). These findings suggest that sesamol has strong potential to protect hematopoietic system by lowering radiation induced DNA damages and can prevent acute hematopoietic syndrome in mice. Environ. Mol. Mutagen., 2017. © 2017 Wiley Periodicals, Inc. |
| تدمد: |
0893-6692 |
| DOI: |
10.1002/em.22118 |
| URL الوصول: |
https://explore.openaire.eu/search/publication?articleId=doi_________::6e803ccc797e6b7bb84ce5bb0e565d34
https://doi.org/10.1002/em.22118 |
| Rights: |
CLOSED |
| رقم الانضمام: |
edsair.doi...........6e803ccc797e6b7bb84ce5bb0e565d34 |
| قاعدة البيانات: |
OpenAIRE |