Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination
| العنوان: | Complete genome sequences of three newly discovered cacao mild mosaic virus isolates from Theobroma cacao L. in Brazil and Puerto Rico and evidence for recombination |
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| المؤلفون: | Judith K. Brown, Jean-Philippe Marelli, Tatsuya Nagata, Osman A. Gutiérrez, Mayra M.M. Ferro, Cory Von Keith, Dahyana Santos Britto, Alina S. Puig, Roberto Ramos-Sobrinho |
| المصدر: | Archives of Virology. 166:2027-2031 |
| بيانات النشر: | Springer Science and Business Media LLC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Mosaic virus, biology, Theobroma, Sequence analysis, General Medicine, biology.organism_classification, Genome, Virology, DNA sequencing, law.invention, law, Human virome, Illumina dye sequencing, Polymerase chain reaction |
| الوصف: | To analyze the DNA virome associated with cacao (Theobroma cacao L.) trees showing virus-like symptoms in Brazil (BR) and Puerto Rico (PR) during 2018-2019, total DNA was isolated from symptomatic leaves and subjected to high-throughput Illumina sequencing. The assembled complete badnaviral genome sequences were verified by PCR amplification, cloning, and DNA sequencing. Based on pairwise distances and phylogenetic analysis, three badnaviral genomes were identified, and these viruses were found to be isolates of the previously described cacao mild mosaic virus (CaMMV). The three genomes were 7,520, 7,524, and 7,514 bp in size for the isolates CaMMV-BR321, CaMMV-BR322, and CaMMV-PR3, respectively. Each genome contained four predicted open reading frames: ORFs 1-3 and ORFY. The CaMMV-PR3 isolate was identified as a probable recombinant, with a CaMMV-BR-like virus as the major parent. |
| تدمد: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s00705-021-05063-5 |
| URL الوصول: | https://explore.openaire.eu/search/publication?articleId=doi_________::64a8d5f6c52e95a2091a615158781788 https://doi.org/10.1007/s00705-021-05063-5 |
| Rights: | CLOSED |
| رقم الانضمام: | edsair.doi...........64a8d5f6c52e95a2091a615158781788 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14328798 03048608 |
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| DOI: | 10.1007/s00705-021-05063-5 |